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TitleDelineating organizational principles of the endogenous L-A virus by cryo-EM and computational analysis of native cell extracts.
Journal, issue, pagesCommun Biol, Vol. 7, Issue 1, Page 557, Year 2024
Publish dateMay 10, 2024
AuthorsLisa Schmidt / Christian Tüting / Fotis L Kyrilis / Farzad Hamdi / Dmitry A Semchonok / Gerd Hause / Annette Meister / Christian Ihling / Milton T Stubbs / Andrea Sinz / Panagiotis L Kastritis /
PubMed AbstractThe high abundance of most viruses in infected host cells benefits their structural characterization. However, endogenous viruses are present in low copy numbers and are therefore challenging to ...The high abundance of most viruses in infected host cells benefits their structural characterization. However, endogenous viruses are present in low copy numbers and are therefore challenging to investigate. Here, we retrieve cell extracts enriched with an endogenous virus, the yeast L-A virus. The determined cryo-EM structure discloses capsid-stabilizing cation-π stacking, widespread across viruses and within the Totiviridae, and an interplay of non-covalent interactions from ten distinct capsomere interfaces. The capsid-embedded mRNA decapping active site trench is supported by a constricting movement of two flexible opposite-facing loops. tRNA-loaded polysomes and other biomacromolecules, presumably mRNA, are found in virus proximity within the cell extract. Mature viruses participate in larger viral communities resembling their rare in-cell equivalents in terms of size, composition, and inter-virus distances. Our results collectively describe a 3D-architecture of a viral milieu, opening the door to cell-extract-based high-resolution structural virology.
External linksCommun Biol / PubMed:38730276 / PubMed Central
MethodsEM (single particle)
Resolution3.21 - 8.1 Å
Structure data

15189

8a5t

EMDB-15189, PDB-8a5t:
Capsid structure of the L-A helper virus from native viral communities
Method: EM (single particle) / Resolution: 3.78 Å

EMDB-15214: Endogenous yeast L-A helper virus identified from native cell extracts
Method: EM (single particle) / Resolution: 6.4 Å

EMDB-15215: Asymmetric reconstruction of averaged ribosomes from Saccharomyces cerevisiae
Method: EM (single particle) / Resolution: 8.1 Å

17628

8pe4

EMDB-17628, PDB-8pe4:
Capsid structure of the L-A helper virus from native viral communities
Method: EM (single particle) / Resolution: 3.21 Å

Source
  • saccharomyces cerevisiae (brewer's yeast)
  • saccharomyces cerevisiae virus l-a
KeywordsVIRUS / Capsid structure ScVLA / viral particle / wildtype / endogenous

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