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-Structure paper
Title | Structural insights into the mechanism of GTP initiation of microtubule assembly. |
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Journal, issue, pages | Nat Commun, Vol. 14, Issue 1, Page 5980, Year 2023 |
Publish date | Sep 25, 2023 |
Authors | Ju Zhou / Anhui Wang / Yinlong Song / Nan Liu / Jia Wang / Yan Li / Xin Liang / Guohui Li / Huiying Chu / Hong-Wei Wang / |
PubMed Abstract | In eukaryotes, the dynamic assembly of microtubules (MT) plays an important role in numerous cellular processes. The underlying mechanism of GTP triggering MT assembly is still unknown. Here, we ...In eukaryotes, the dynamic assembly of microtubules (MT) plays an important role in numerous cellular processes. The underlying mechanism of GTP triggering MT assembly is still unknown. Here, we present cryo-EM structures of tubulin heterodimer at their GTP- and GDP-bound states, intermediate assembly states of GTP-tubulin, and final assembly stages of MT. Both GTP- and GDP-tubulin heterodimers adopt similar curved conformations with subtle flexibility differences. In head-to-tail oligomers of tubulin heterodimers, the inter-dimer interface of GDP-tubulin exhibits greater flexibility, particularly in tangential bending. Cryo-EM of the intermediate assembly states reveals two types of tubulin lateral contacts, "Tube-bond" and "MT-bond". Further, molecular dynamics (MD) simulations show that GTP triggers lateral contact formation in MT assembly in multiple sequential steps, gradually straightening the curved tubulin heterodimers. Therefore, we propose a flexible model of GTP-initiated MT assembly, including the formation of longitudinal and lateral contacts, to explain the nucleation and assembly of MT. |
External links | Nat Commun / PubMed:37749104 / PubMed Central |
Methods | EM (single particle) / EM (helical sym.) |
Resolution | 3.5 - 6.8 Å |
Structure data | EMDB-34077, PDB-7ysn: EMDB-34078, PDB-7yso: EMDB-34079, PDB-7ysp: EMDB-34080, PDB-7ysq: EMDB-34081, PDB-7ysr: |
Chemicals | ChemComp-GTP: ChemComp-G2P: ChemComp-GDP: ChemComp-GSP: |
Source |
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Keywords | STRUCTURAL PROTEIN / cytoskeleton / PROTEIN FIBRIL / Complex |