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Structure paper

TitleStructural basis for intrinsic transcription termination.
Journal, issue, pagesNature, Vol. 613, Issue 7945, Page 783-789, Year 2023
Publish dateJan 11, 2023
AuthorsLinlin You / Expery O Omollo / Chengzhi Yu / Rachel A Mooney / Jing Shi / Liqiang Shen / Xiaoxian Wu / Aijia Wen / Dingwei He / Yuan Zeng / Yu Feng / Robert Landick / Yu Zhang /
PubMed AbstractEfficient and accurate termination is required for gene transcription in all living organisms. Cellular RNA polymerases in both bacteria and eukaryotes can terminate their transcription through a ...Efficient and accurate termination is required for gene transcription in all living organisms. Cellular RNA polymerases in both bacteria and eukaryotes can terminate their transcription through a factor-independent termination pathway-called intrinsic termination transcription in bacteria-in which RNA polymerase recognizes terminator sequences, stops nucleotide addition and releases nascent RNA spontaneously. Here we report a set of single-particle cryo-electron microscopy structures of Escherichia coli transcription intrinsic termination complexes representing key intermediate states of the event. The structures show how RNA polymerase pauses at terminator sequences, how the terminator RNA hairpin folds inside RNA polymerase, and how RNA polymerase rewinds the transcription bubble to release RNA and then DNA. These macromolecular snapshots define a structural mechanism for bacterial intrinsic termination and a pathway for RNA release and DNA collapse that is relevant for factor-independent termination by all RNA polymerases.
External linksNature / PubMed:36631609 / PubMed Central
MethodsEM (single particle)
Resolution3.05 - 3.58 Å
Structure data

33996

7yp9

EMDB-33996, PDB-7yp9:
Cryo-EM structure of Escherichia coli paused complex of transcription termination (TTC-pause)
Method: EM (single particle) / Resolution: 3.58 Å

33997

7ypa

EMDB-33997, PDB-7ypa:
Cryo-EM structure of Escherichia coli hairpin-nucleation complex of transcription termination (TTC-hairpin)
Method: EM (single particle) / Resolution: 3.05 Å

33998

7ypb

EMDB-33998, PDB-7ypb:
Cryo-EM structure of Escherichia coli release complex of transcription termination (TTC-release)
Method: EM (single particle) / Resolution: 3.48 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-MG:
Unknown entry

Source
  • escherichia coli k-12 (bacteria)
  • synthetic construct (others)
KeywordsTRANSCRIPTION/DNA/RNA / Termination / Intrinsic termination / U-tract pause / Transcription pause / Factor-independent termination / TRANSCRIPTION-DNA-RNA COMPLEX / Hairpin nucleation / Pre-termination / TRANSCRIPTION//DNA/RNA / RNA release / Transcription bubble rewinding / TRANSCRIPTION--DNA-RNA complex

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