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TitleCryo-EM structure of the nuclear ring from Xenopus laevis nuclear pore complex.
Journal, issue, pagesCell Res, Vol. 32, Issue 4, Page 349-358, Year 2022
Publish dateFeb 17, 2022
AuthorsGaoxingyu Huang / Xiechao Zhan / Chao Zeng / Xuechen Zhu / Ke Liang / Yanyu Zhao / Pan Wang / Qifan Wang / Qiang Zhou / Qinghua Tao / Minhao Liu / Jianlin Lei / Chuangye Yan / Yigong Shi /
PubMed AbstractNuclear pore complex (NPC) shuttles cargo across the nuclear envelope. Here we present single-particle cryo-EM structure of the nuclear ring (NR) subunit from Xenopus laevis NPC at an average ...Nuclear pore complex (NPC) shuttles cargo across the nuclear envelope. Here we present single-particle cryo-EM structure of the nuclear ring (NR) subunit from Xenopus laevis NPC at an average resolution of 5.6 Å. The NR subunit comprises two 10-membered Y complexes, each with the nucleoporin ELYS closely associating with Nup160 and Nup37 of the long arm. Unlike the cytoplasmic ring (CR) or inner ring (IR), the NR subunit contains only one molecule each of Nup205 and Nup93. Nup205 binds both arms of the Y complexes and interacts with the stem of inner Y complex from the neighboring subunit. Nup93 connects the stems of inner and outer Y complexes within the same NR subunit, and places its N-terminal extended helix into the axial groove of Nup205 from the neighboring subunit. Together with other structural information, we have generated a composite atomic model of the central ring scaffold that includes the NR, IR, and CR. The IR is connected to the two outer rings mainly through Nup155. This model facilitates functional understanding of vertebrate NPC.
External linksCell Res / PubMed:35177819 / PubMed Central
MethodsEM (single particle)
Resolution5.6 Å
Structure data

EMDB-32394, PDB-7wb4:
Cryo-EM structure of the NR subunit from X. laevis NPC
Method: EM (single particle) / Resolution: 5.6 Å

Source
  • xenopus laevis (African clawed frog)
KeywordsSTRUCTURAL PROTEIN / nuclear pore complex / nuclear ring / Nup205 / Nup93 / Y complex / Elys

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