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- EMDB-32394: Cryo-EM structure of the NR subunit from X. laevis NPC -

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Basic information

Entry
Database: EMDB / ID: EMD-32394
TitleCryo-EM structure of the NR subunit from X. laevis NPC
Map dataThe EM map of NR subunit
Sample
  • Complex: The NR subunit
    • Protein or peptide: x 13 types
Keywordsnuclear pore complex / nuclear ring / Nup205 / Nup93 / Y complex / Elys / STRUCTURAL PROTEIN
Function / homology
Function and homology information


: / nitrogen compound transport / macromolecule localization / GATOR2 complex / nephron development / macromolecule metabolic process / nuclear pore organization / nuclear pore outer ring / nuclear pore complex assembly / attachment of mitotic spindle microtubules to kinetochore ...: / nitrogen compound transport / macromolecule localization / GATOR2 complex / nephron development / macromolecule metabolic process / nuclear pore organization / nuclear pore outer ring / nuclear pore complex assembly / attachment of mitotic spindle microtubules to kinetochore / structural constituent of nuclear pore / nucleocytoplasmic transport / mitotic metaphase chromosome alignment / mRNA transport / cellular response to nutrient levels / nuclear pore / positive regulation of TORC1 signaling / nuclear periphery / kinetochore / protein transport / nuclear membrane / cell division / lysosomal membrane / structural molecule activity / DNA binding / nucleoplasm / cytosol / cytoplasm
Similarity search - Function
Nuclear pore complex protein NUP98-NUP96 / ELYS-like domain / ELYS, beta-propeller domain / : / Nuclear pore complex assembly / beta-propeller of ELYS nucleoporin / Nucleoporin, Nup155-like, C-terminal, subdomain 3 / Nucleoporin Nup37 / Nucleoporin Nup85-like / Nucleoporin Nup120/160 ...Nuclear pore complex protein NUP98-NUP96 / ELYS-like domain / ELYS, beta-propeller domain / : / Nuclear pore complex assembly / beta-propeller of ELYS nucleoporin / Nucleoporin, Nup155-like, C-terminal, subdomain 3 / Nucleoporin Nup37 / Nucleoporin Nup85-like / Nucleoporin Nup120/160 / Nup85 Nucleoporin / Nuclear pore protein 84/107 / Nuclear pore protein 84 / 107 / Nuclear pore complex protein Nup133-like / Nucleoporin, Nup155-like / Nucleoporin, Nup155-like, C-terminal, subdomain 1 / Nucleoporin, Nup155-like, C-terminal, subdomain 2 / Nucleoporin Nup186/Nup192/Nup205 / Nuclear pore complex scaffold, nucleoporins 186/192/205 / Nucleoporin, Nup133/Nup155-like, C-terminal / Non-repetitive/WGA-negative nucleoporin C-terminal / Nucleoporin interacting component Nup93/Nic96 / Nup93/Nic96 / Nucleoporin FG repeat / Nucleoporin FG repeat region / Nucleoporin, Nup133/Nup155-like, N-terminal / Nup133 N terminal like / Sec13/Seh1 family / Nuclear pore complex protein NUP96, C-terminal domain / Nuclear protein 96 / Nuclear pore complex protein Nup98-Nup96-like, autopeptidase S59 domain / Nuclear pore complex protein Nup98-Nup96-like, autopeptidase S59 domain superfamily / Nucleoporin peptidase S59-like / Nucleoporin autopeptidase / NUP C-terminal domain profile. / Quinoprotein alcohol dehydrogenase-like superfamily / G-protein beta WD-40 repeat / WD40 repeat, conserved site / Trp-Asp (WD) repeats signature. / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD domain, G-beta repeat / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily
Similarity search - Domain/homology
Uncharacterized protein / Nuclear pore complex protein Nup133 / Nuclear pore complex protein Nup98-Nup96 / Nuclear pore complex protein / MGC154553 protein / Protein ELYS / MGC83295 protein / MGC83926 protein / Nuclear pore complex protein Nup85 / Nucleoporin SEH1-B ...Uncharacterized protein / Nuclear pore complex protein Nup133 / Nuclear pore complex protein Nup98-Nup96 / Nuclear pore complex protein / MGC154553 protein / Protein ELYS / MGC83295 protein / MGC83926 protein / Nuclear pore complex protein Nup85 / Nucleoporin SEH1-B / Nucleoporin 155kDa L homeolog / Nuclear pore complex protein Nup93 / Protein SEC13 homolog
Similarity search - Component
Biological speciesXenopus laevis (African clawed frog)
Methodsingle particle reconstruction / cryo EM / Resolution: 5.6 Å
AuthorsHuang G / Zhan X
Funding support China, 1 items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC) China
CitationJournal: Cell Res / Year: 2022
Title: Cryo-EM structure of the nuclear ring from Xenopus laevis nuclear pore complex.
Authors: Gaoxingyu Huang / Xiechao Zhan / Chao Zeng / Xuechen Zhu / Ke Liang / Yanyu Zhao / Pan Wang / Qifan Wang / Qiang Zhou / Qinghua Tao / Minhao Liu / Jianlin Lei / Chuangye Yan / Yigong Shi /
Abstract: Nuclear pore complex (NPC) shuttles cargo across the nuclear envelope. Here we present single-particle cryo-EM structure of the nuclear ring (NR) subunit from Xenopus laevis NPC at an average ...Nuclear pore complex (NPC) shuttles cargo across the nuclear envelope. Here we present single-particle cryo-EM structure of the nuclear ring (NR) subunit from Xenopus laevis NPC at an average resolution of 5.6 Å. The NR subunit comprises two 10-membered Y complexes, each with the nucleoporin ELYS closely associating with Nup160 and Nup37 of the long arm. Unlike the cytoplasmic ring (CR) or inner ring (IR), the NR subunit contains only one molecule each of Nup205 and Nup93. Nup205 binds both arms of the Y complexes and interacts with the stem of inner Y complex from the neighboring subunit. Nup93 connects the stems of inner and outer Y complexes within the same NR subunit, and places its N-terminal extended helix into the axial groove of Nup205 from the neighboring subunit. Together with other structural information, we have generated a composite atomic model of the central ring scaffold that includes the NR, IR, and CR. The IR is connected to the two outer rings mainly through Nup155. This model facilitates functional understanding of vertebrate NPC.
History
DepositionDec 15, 2021-
Header (metadata) releaseMar 2, 2022-
Map releaseMar 2, 2022-
UpdateJun 26, 2024-
Current statusJun 26, 2024Processing site: PDBj / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.006
  • Imaged by UCSF Chimera
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  • Surface view colored by radius
  • Surface level: 0.006
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-7wb4
  • Surface level: 0.006
  • Imaged by UCSF Chimera
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  • Simplified surface model + fitted atomic model
  • Atomic modelsPDB-7wb4
  • Imaged by Jmol
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_32394.png

Downloads & links

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Map

FileDownload / File: emd_32394.map.gz / Format: CCP4 / Size: 30.5 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationThe EM map of NR subunit
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
2.77 Å/pix.
x 200 pix.
= 554.8 Å		2.77 Å/pix.
x 200 pix.
= 554.8 Å		2.77 Å/pix.
x 200 pix.
= 554.8 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 2.774 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.006 / Movie #1: 0.006
Minimum - Maximum-0.01828787 - 0.04477132
Average (Standard dev.)-0.000046797704 (±0.0021272595)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions200200200
Spacing200200200
CellA=B=C: 554.8 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z2.7742.7742.774
M x/y/z200200200
origin x/y/z0.0000.0000.000
length x/y/z554.800554.800554.800
α/β/γ90.00090.00090.000
start NX/NY/NZ535455
NX/NY/NZ134138134
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS200200200
D min/max/mean-0.0180.045-0.000

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Supplemental data

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Sample components

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Entire : The NR subunit

EntireName: The NR subunit
Components
  • Complex: The NR subunit
    • Protein or peptide: outer Nup133
    • Protein or peptide: Nuclear pore complex protein Nup85
    • Protein or peptide: MGC154553 protein
    • Protein or peptide: MGC83926 protein
    • Protein or peptide: Nucleoporin SEH1-B
    • Protein or peptide: outer Nup160
    • Protein or peptide: Nup155-prov protein
    • Protein or peptide: GATOR complex protein SEC13
    • Protein or peptide: Nuclear pore complex protein Nup96
    • Protein or peptide: Nuclear pore complex protein
    • Protein or peptide: Nuclear pore complex protein Nup93
    • Protein or peptide: MGC83295 protein
    • Protein or peptide: Protein ELYS

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Supramolecule #1: The NR subunit

SupramoleculeName: The NR subunit / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Xenopus laevis (African clawed frog)

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Macromolecule #1: outer Nup133

MacromoleculeName: outer Nup133 / type: protein_or_peptide / ID: 1 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Xenopus laevis (African clawed frog)
Molecular weightTheoretical: 127.55125 KDa
SequenceString: MFPSPRAQGM GSARRPFNSR LTGGRKALGP GVTASSSPSA LYSPVGRRVS ASGARSTPSR VYLHPAASET VNYNVQLFGS SLPVKVMEA LSNASADEPM AACIHEGGWA WLACNDRLII WKISHSSSAK LMVCKELPLP LSDSEWSADL VDICAQTGDP A AAQSVALM ...String:
MFPSPRAQGM GSARRPFNSR LTGGRKALGP GVTASSSPSA LYSPVGRRVS ASGARSTPSR VYLHPAASET VNYNVQLFGS SLPVKVMEA LSNASADEPM AACIHEGGWA WLACNDRLII WKISHSSSAK LMVCKELPLP LSDSEWSADL VDICAQTGDP A AAQSVALM AATPEGSSRY WPNILHEGTY IESYTEFGSS LCAFVTAVKG NSFILSSEKN QLVRLTPDAS GKMNQRVLPQ GQ GMLSGIG RRVSTLFGIL SPAVESTLCS VLWDKGDCFY TLTDSSINKW DLDDTSESQV LNWDMSRVLR EYISDAIWGS ESD YDDIKA GININYLSLN QNCDGLVILS AAWHPGDNPC QIYYTLVTVK DEGYNISDEI TVEVTQFNPV FQARGMQLCQ LVVP NFSSQ ACYLYTQEMI FACSTGTGRS TLPQEKIPFE AQGDNIVGAG SCEGWPVFFI RKSGMLTVVA RETASVLPEH MEESL SSVS KSSRQAVVKD SRPDQIAHDD KTKHLKAAFL RYCRKDILGA QSMVDSLFSD SDMEPDDELD LAVNQISVDL IDDYPA SDP RWAESVPEEA AGFSNTSLIL LHQLEDKMKA HSFFVDFLHQ VGLFSRLSTC QTKGMLVATR LLLSEHAEKL SAAIVLK NH HAKLPVLVNS AIQLALDKRM CTVPQNLTAA DVYFREVSQM EIIFECLVDK EEADLESTSI DSVEWANIVV NVNTILKD M LHVACQYRQS KNSLYKNESG IQEPEHVPWT ASSGTAGIRS VVTRQHGIIL KVYPQADSGL RTILIEQLAA LLNYLLDDY VTQLKSIDKL ANEERYNILE MEYAQKRSEL LSPLLILGQY AWASNLAEKY CDFDILVQIC EMTDNQSRLQ RYMTLFAEQN FSDFLFRWY LEKGKRGKLL SQPASQHGQL AAFLQAHDHL SWLHELNSQE FEKAHRTLQT LANMETRYFC KKKTLLGLSK L AALASDFQ EDVLQEKVEE IAEQEHFLLH QETLPKKLLE EKQLDLNAMP VLAPFQLIQL YVCEENKRAN ENDFMKALDL LE YIGDDSE VDVEELKLEI LCKAIKRDEW SATDGKDDPI EATKDSIFVK VLQNLLNKGI ELKGYLPKAE TLLQSEELNS LKT NSYFEF SLKANYECYM KMQS

UniProtKB: Nuclear pore complex protein Nup133

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Macromolecule #2: Nuclear pore complex protein Nup85

MacromoleculeName: Nuclear pore complex protein Nup85 / type: protein_or_peptide / ID: 2 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Xenopus laevis (African clawed frog)
Molecular weightTheoretical: 75.160047 KDa
SequenceString: MEELDVDPAE TPIPGLGQQN RHIGFSWGPG DLLLYETLYQ KQGNSETAAR CPFMYLVRSD EDIYSPVLRK LFNESHSIFV GLQKSAEEA SGKSRKAQLV QVSRNYRSVL RACMEEMHTL SESTRETAQK YISQISILSA MELSWNLCEI LFIESAPAGP L LILLLEWV ...String:
MEELDVDPAE TPIPGLGQQN RHIGFSWGPG DLLLYETLYQ KQGNSETAAR CPFMYLVRSD EDIYSPVLRK LFNESHSIFV GLQKSAEEA SGKSRKAQLV QVSRNYRSVL RACMEEMHTL SESTRETAQK YISQISILSA MELSWNLCEI LFIESAPAGP L LILLLEWV RLHVCEVDNI VQDVLRSEKP TEHEKFWDGV TGYVLQGRMN EARQLLAKEA STSASARSMC RVLDDLLKKM PM LHTGGTQ TLTEFELKWQ HWREECERHL QNGTFSSNVH MEAVCRVLLG DEEVLLEKRD LMTTWYHFLV SRLLFKHPTV KPT ELHFYA QSSLDMFLAG DSCPEPLDNI LLAAFEFDIH QVIKEFSIVS SNWWFVAHLT DLLDHCQLFQ AHNLYFGANM REFL LLDYA SGLFSHHSLW QLGVDYFDYC PNLGREYLKL HMERIPLSTE KKALKALRIC EQRQMTEQVR SICKTMAMQS LCNRR LGSA LSWSIRAKDA AFATLISDRF LKEYCERGNF TDLDLIDNLG SAMLLSDRLT FLGKYREFHR MYSQEQFSEA ASLLLS LMT ARIAPCSFWL TLLLDALPLL EQKQVIFSAE QTYELMRCLE DRMAAKLEST SPDEIQKQDS SIDNTKVEML RLALARN LA RAIVTEGALQ E

UniProtKB: Nuclear pore complex protein Nup85

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Macromolecule #3: MGC154553 protein

MacromoleculeName: MGC154553 protein / type: protein_or_peptide / ID: 3 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Xenopus laevis (African clawed frog)
Molecular weightTheoretical: 41.744512 KDa
SequenceString: MADKFAAKFV SHKISRTRWR PVSASSLQQP DVFATGSWDN EENKVCVWAT SDFGATSLDE EYQGDPKQLC DIKHPGDVMD MQFLDKERI VTGSSTGTVT IFRHHENNQT LSVNQRWEQA HYHVGSNMRA PCTAIVCSSP EIVSVGEDGR INCFRAESRD V LRTIDDAD ...String:
MADKFAAKFV SHKISRTRWR PVSASSLQQP DVFATGSWDN EENKVCVWAT SDFGATSLDE EYQGDPKQLC DIKHPGDVMD MQFLDKERI VTGSSTGTVT IFRHHENNQT LSVNQRWEQA HYHVGSNMRA PCTAIVCSSP EIVSVGEDGR INCFRAESRD V LRTIDDAD SSTMHGVTFL RTTEILTVNS VGQLKLWDLR KQGNDPTQIF SVTGERVPLH CVDRHPNQQH VVATGGQDGM LC IWDVRHG KMPMSLLNAH EAEMWEVHFH PSNPDHLFTC SEDGSLWHWD ASADSEKPTF LLGGRSTFNI SRSSIAPPNA NQS LACAWL STDPTKGQLE ITNLLPSSTL SVNSLDVLGQ NLVCGTDAEA IYVTRRLFS

UniProtKB: MGC154553 protein

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Macromolecule #4: MGC83926 protein

MacromoleculeName: MGC83926 protein / type: protein_or_peptide / ID: 4 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Xenopus laevis (African clawed frog)
Molecular weightTheoretical: 36.588625 KDa
SequenceString: MKQDSASNAT YTVDCEDYVH VVEFNPFDSG EAGSLLAYGG ISYVVIASCR FQEEDSTVEG IEFKTLKTFH HGERVVAIAW SPETRCDAL LPLLRFATAA GDKKIRIFTS DFQDKNEYKV IEGHSGYIND LVFCSPEGTD IASVGDDHTC RIWDLDGKQI A MFILRSPG ...String:
MKQDSASNAT YTVDCEDYVH VVEFNPFDSG EAGSLLAYGG ISYVVIASCR FQEEDSTVEG IEFKTLKTFH HGERVVAIAW SPETRCDAL LPLLRFATAA GDKKIRIFTS DFQDKNEYKV IEGHSGYIND LVFCSPEGTD IASVGDDHTC RIWDLDGKQI A MFILRSPG MSVAWHPEGA FKLMVAEKTG TIRFYDLTTH QAILSLESVQ VPLMSADWCV RNTLRIGAVA GNDWIIWEMP RS SYPQDNK PAHADRARMF RWSKCNENVF ATTGYPGKMK SQIAIHHLAH PQPILIGTAP VGSGLSWHRR LPLCVVGGYR KLF FWLTEM

UniProtKB: MGC83926 protein

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Macromolecule #5: Nucleoporin SEH1-B

MacromoleculeName: Nucleoporin SEH1-B / type: protein_or_peptide / ID: 5 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Xenopus laevis (African clawed frog)
Molecular weightTheoretical: 39.798602 KDa
SequenceString: MFVARSIAAD HKDLIHDVSF DFHGRRMATC SSDQSVKVWD KSENVNWHCT ASWKTHSGSV WRVTWAHPEF GQVLASCSFD RTAAVWEEI VGESNDKLRG QSHWVKRTTL VDSRTSVTDV KFAPKHMGLM LATCSADGVV RIYEAPDVMN LSQWSLQHEI S CKLSCSCI ...String:
MFVARSIAAD HKDLIHDVSF DFHGRRMATC SSDQSVKVWD KSENVNWHCT ASWKTHSGSV WRVTWAHPEF GQVLASCSFD RTAAVWEEI VGESNDKLRG QSHWVKRTTL VDSRTSVTDV KFAPKHMGLM LATCSADGVV RIYEAPDVMN LSQWSLQHEI S CKLSCSCI SWNPSSSRAH SPMIAVGSDD SSPNIMGKVQ IYEYNENTRK YAKAETLMSV SDPVHDIAFA PNLGRSFHIL AV ATKDVRI FTMKPLRKEL SSSGGVTKFE NHTVAQFDNH NSQVWRVSWN ITGTVLASSG DDGTVRLWKA NYMDNWKCIG VLK GDGNPV GNSFQGIFGS SIGSASHGLQ NSVNGTSTSG RKHS

UniProtKB: Nucleoporin SEH1-B

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Macromolecule #6: outer Nup160

MacromoleculeName: outer Nup160 / type: protein_or_peptide / ID: 6 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Xenopus laevis (African clawed frog)
Molecular weightTheoretical: 162.658234 KDa
SequenceString: MAAAERHMTP FQAIDWAGSI TLPMVQRVGG FTRAIMAASV NLERSYMELI GAERETSRRN FRDLSLRPDV NLVIGGPKYA DCAGGYCYS ESSSLLSATR NRFLHWTSYA DTLELVEISL DINLVNNAVR LRILNCSILP GGVHICETPN NIVVLILTNQ T VHRLILPH ...String:
MAAAERHMTP FQAIDWAGSI TLPMVQRVGG FTRAIMAASV NLERSYMELI GAERETSRRN FRDLSLRPDV NLVIGGPKYA DCAGGYCYS ESSSLLSATR NRFLHWTSYA DTLELVEISL DINLVNNAVR LRILNCSILP GGVHICETPN NIVVLILTNQ T VHRLILPH PSRMYRSEII SDSHIQSIFT DIGKTNFHDP SNTYVIPAIP GRAPNTTAST AWLSSDGEAL FALPSISGGI LV IKMPPHD MEGLVTIAEL KQSSVMQRLL TGWMPSSIRG DQGPAHLPVS LAVHTLDHDS YLFALCQDHK LRMWSYKDQM CLM VADMLE YVPVSKDIRQ TAGTGHKLRL AFSETLGILY LGVYLHTPKQ GQFCVFQLMC AESNRYSLDH ISSIFTNQET LIDF TFTLT SMDIWALWLD DDNQTVVKHI NFEENQAGQW NPVFVNPLPE DDLAISDEQE PQEAYLECLF APGRFTIAAV QKAIQ ILRK GSGRVLDLSW EELRKDVTLT VENEIQNAVI DYDVSQEEFR QINIENWCKF YTCCLQYQET LSRPLALLVH PDTNMV CLL RKGFLSFLAP CSLVEHLYLV PAEHLLTVDE SVISDDIDAA SDIVNLIQCL RMIADYISED MAYLMESACC HLQSPER VA EQILEDLIAN DIDNIMENIQ NKLQDTRNPI RAIGFLLQNM DYETNADMEQ PQPNTRLNLS TLYGSITASS VVCQAICK I SATRFLICRD LLILQHLLLR LGDMALIGAG QLLHSQQELI PRAAQLLLSY YMIRWGSQCL ACAVPVDILE SNLQHLSVL ELSDSQVEKR RYTSGIQTIV ELFFEDVARK HFPHVFIQSG ASQLQEPLNW SDLIKRITNY LLQLLWPSNP NFQFAECLMR NCQYTQLQE YVRLLLPWCQ VNVGSCHFML AQCYLVAGEG HKALDCFSQA ASEVEREDFL EKLIRVEEGE SVSPRLQYYN R VLRLLEDV GLPELVIQLA TIAIGEASDD WRSQAALRTR IFKHHLDMGH NNQAYDALTQ IPDPSRQLDC LRQLVVVLCE RS QLQDLVE FPYVNLHNEV VGIIESRARA VDLMTHNYYE LLYAFHIYRH NYRKAGSVMF EYGMRLGREV RTLRGLQKQV NSY LACLNC LRLIRPEYAW IVQPVSGAVY ERPGASPKRN YDGESSAVPS SSQIEILELR DLEKEYVLAQ TRLTLAKHNP STAA IAGSS AAEEMVALLV QAGLFDTAIS LCQTFKLALT SVFEGLACKC IRLQQGGEAA QAEAWEWLAA NQLATVITTK ESSAT DEAW RLMISYLDKY EAKNTLYHHC IINKLLSHGV PLPNWLINRY KAMDAAELLR LYLKYDLLEE AAELVLEYVD ALLGKG HQY FGIQAPLSAT SQLVWFPYSA IDHLRQALGE NESNQHNQAI LSKLQRKMDE YFQKLKKATD DYKKLVQKPL RA

UniProtKB: Uncharacterized protein

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Macromolecule #7: Nup155-prov protein

MacromoleculeName: Nup155-prov protein / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xenopus laevis (African clawed frog)
Molecular weightTheoretical: 154.922422 KDa
SequenceString: MPSATPGAPA ASAAMQEALE GAGRIIDRLL QEDRAYPDLS ELLNVPVHTC PTISGVSEMD YPLQSPGLLT IPSLPEISAI RRVPLPPEL IEQFGHMQCN CMMGVFPEIS RAWLTIDSDI FMWNYEDGGD LAYYDGLSET ILSVGLVKPK TGIFQPHIRF L LVLSTPVD ...String:
MPSATPGAPA ASAAMQEALE GAGRIIDRLL QEDRAYPDLS ELLNVPVHTC PTISGVSEMD YPLQSPGLLT IPSLPEISAI RRVPLPPEL IEQFGHMQCN CMMGVFPEIS RAWLTIDSDI FMWNYEDGGD LAYYDGLSET ILSVGLVKPK TGIFQPHIRF L LVLSTPVD IVILGLSFAN LQPGNLNDSI SGGMQLLPDP LYSLPTDNTY LLSITSTDNG RIFLSGKDGC LYEVEYQAEA GW FSQRCRK INHSKSSLSF LVPSVLQFAF SEDDPIVQIA IDNSRNILYT RSEKGVIQVY DLGVDGHGMS RVASVSQNSL VSA AGNIAR TIDRNVFKPI IHISVIEMSE SVNCHLLAVT HTGVRFYFST VPFKQPTARP CMLALVHVRL PPGFSASSNV EKPS KVHKA LYNNGVLLMA ASENEDNDML WCINRDSFPF QKPMMETQVT TQVDGHSWAL SAVDEQKADK IVTPLNKDLI PLTDS PVII QQHMIPPKRF VLLSAQGSHI FYKLRPVDQL RHLLVSNSGG DGEEIERFFK LHQENQACAT CLILACSSAA SDREVS SWA ARAFFRYGGE AQLRVQSALH APSNVGPIFG SPLPVASPIP VGSPMPNPSF LGTPTQGACP PNVSTPAYGV ATPAPQP PA VPGMMGTEIV FSGKHNGICI YFCRIIGNIW DGSVAVENTF QSGNREVTAI DSSVTPQHLE SVLKELKGLL EFLDRYSQ F TAGSLGNPGF GTPANRQQRL VSLGRPDIGS SQQAQQELQR KYHTEAQLAE QFSLQGIHQL VRKMCQALAL WKLLCEHQF SLVVSDLQKE LQEQLKITTF KDLVIRDKEL TGALTASLIS CYIRDNASVD GISYRLQEVC PLLYSTDDAV CSKANELLQR SRHVPNKQE KERMLRESLK EYQKISQQVD LPNVCAQYRQ VRFYEGVVEL CLSAAEKKDP QGLGLHFHKN GEPEEDMAGL Q AFQERLNS YKCITDTLQE LVNQSKAAPQ SPSVPKKPGP PVLSSDPNML SNEEAGIHFE QMLKLAQRSA DELFNIALFN WL IQADLTD KLLELNSPFL EPHLVRMAKL DQNKVRYMDL LWRYYEKNRN FSNAARVVAK LADMHSPEIS LKQRLEYISR AIL SAKSST TMSTLAADGE FLHELEEKLE VARIQLQIQE TLTRQYSHHS TVGDAVSQLD SQLMDITKMF GQYADPFRLS ECKL AIIHC AGHSDPILVQ TLWQDIIDKE LSDSMGNSSV DRMQSLHLKI TSLGKIYAST PRYFPLEFLV KYLEQQVCNF SWDAG FVTY TMQEINVPVP KLLEVYDHLF KARDPWWSRM KKPLHLLESI YILLSGYVQE PSKVPSYERR RFTTLCLDAI SCYLVE LQS MDPAPALLNT VSNFKSLQAK LERLS

UniProtKB: Nucleoporin 155kDa L homeolog

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Macromolecule #8: GATOR complex protein SEC13

MacromoleculeName: GATOR complex protein SEC13 / type: protein_or_peptide / ID: 8 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Xenopus laevis (African clawed frog)
Molecular weightTheoretical: 35.315285 KDa
SequenceString: MVSVINTVDT SHEDMIHDAQ MDYYGIRLAT CSSDRSVKIF DVKNGGQILI ADLRGHDGPV WQVAWAHPMY GNILASCSYD RKVIIWKEE NGTWEKTYEY TGHDSSVNSV CWAPHDFGLV LACGSSDGAI SILTFTGDGP WEVKKISNAH TIGCNAVSWA P SVIPGSLV ...String:
MVSVINTVDT SHEDMIHDAQ MDYYGIRLAT CSSDRSVKIF DVKNGGQILI ADLRGHDGPV WQVAWAHPMY GNILASCSYD RKVIIWKEE NGTWEKTYEY TGHDSSVNSV CWAPHDFGLV LACGSSDGAI SILTFTGDGP WEVKKISNAH TIGCNAVSWA P SVIPGSLV DQPSSQKPNY IKRFVSGGCD NLVKIWREED GQWKEDQKLE AHSDWVRDVA WAPSIGLPTS TIASCSQDGR VY IWTSDDA ATNCWTPKLL HKFNDVVWHV SWSITANILA VSGGDNKVTL WKESVDGQWA CISDVNKGQG AVSTVTEGQL NDQ

UniProtKB: Protein SEC13 homolog

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Macromolecule #9: Nuclear pore complex protein Nup96

MacromoleculeName: Nuclear pore complex protein Nup96 / type: protein_or_peptide / ID: 9 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Xenopus laevis (African clawed frog)
Molecular weightTheoretical: 105.079148 KDa
SequenceString: SKYGLQDSDE EDDQLNNAEA KKLKAAPVPP QGKPPPLQQA TLPGKVTPPP QSPAVDQLDR VLELDSDMAD ITQDQDLDSV AEEQDITEE QEPLSASSHI ASSLGINPHA LQVMKASLLL EEEDGEMINR FSSFPSSMDP YPDVRSPRLF PSSHAKRTSS M GLLQSKFA ...String:
SKYGLQDSDE EDDQLNNAEA KKLKAAPVPP QGKPPPLQQA TLPGKVTPPP QSPAVDQLDR VLELDSDMAD ITQDQDLDSV AEEQDITEE QEPLSASSHI ASSLGINPHA LQVMKASLLL EEEDGEMINR FSSFPSSMDP YPDVRSPRLF PSSHAKRTSS M GLLQSKFA SPSISRISET AQGSHSPRIL PVTPWSVPAP LAPTFVIPRP APETHLRTVG TRRQQELVPL EKSVTHGRGS LL IDMGLFM GRSFRVGWGP NWTLVHNGDK LTERLNAEED QNMDTIDYGF LPKPTSAKSL TESPFKVHME KLSLEQKSRE LQS YLMPLE IELKNSSVDR SAQCPHFKPN AGVAAIHDYA GWVRNLSNEA GELEAVVKQW GLTWTLCESL WGQLKELEAS LDEP NEYVR NLERRKAFSH WLAHTAEERI EEEVSLYGPE RHVEAVFSFL TGGRISDACR LAQKSGDHRL SLLLSQMVGS QEMRE LISL QLVDWNKLQV DHYIQEERLR VFCLLSGTPV WRSSDNRSIN VCSQLDWKRT LAVHLWYMLP PTATIAQALR LYERAF QEH EEGEPYACYP LPPYLEDCSI SLGDEPSAKF SSLQRDVCVH LLKLYSERQY DLCQLLDPSS VTPDPLDYRL SWHLWMV LQ ALNYTHLSEH RQGTLHASYA AQLENVGLWE WAIFVLLHIP HPHIREAGVR ELLNRQCVVR ESPESLAKEN FLIHRLCV P AQWVHEAKAI RSRRDGDRHK EALYLLKGHQ WNPCHKLVTR HLAADAVINE NYRYLQSFLG ELSNPEHCKH IQDWETAGK VYLDYIRVID MLNLIQQDES SGCELEKLHT KVMSLCKWVE LIHCYTAKDR LAQSEMAKRV ANILRVVLSL QQPPESMSDS SEPRVPLRL LAPHIGRLPM PEDYALEELR GLTQSYLREL ICDS

UniProtKB: Nuclear pore complex protein Nup98-Nup96

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Macromolecule #10: Nuclear pore complex protein

MacromoleculeName: Nuclear pore complex protein / type: protein_or_peptide / ID: 10 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Xenopus laevis (African clawed frog)
Molecular weightTheoretical: 105.398547 KDa
SequenceString: MDMLSPVVRE AEVSRAARRQ SSNRKNPADE SWSNATPTRG PSSRTTGQTL FRQHMTPQTW NSSRPPDVSA ILGTVGRSPR LLQTPGRLA NLSMMSNPDD SVWTTTFSPG RTGMYTTLDS PSFTEDITLS AVMLQEEDPG EAATMSMYPD FLKSFLEHPS S AVFELIEQ ...String:
MDMLSPVVRE AEVSRAARRQ SSNRKNPADE SWSNATPTRG PSSRTTGQTL FRQHMTPQTW NSSRPPDVSA ILGTVGRSPR LLQTPGRLA NLSMMSNPDD SVWTTTFSPG RTGMYTTLDS PSFTEDITLS AVMLQEEDPG EAATMSMYPD FLKSFLEHPS S AVFELIEQ YEATCNTQIT LLKKIVKRVT PGQQKFSKTA SILWLLQQEM VTWRLIAALY RDRIQSALEE ENMFEIAAPN AS EKTIVDK LFQRDTLVRQ SQLVVDWLES IAKDEVGDFS DNIEYYAKSV YWENTLHTLK QRSMLSLGSS RPLVSELDPD API RQKLPL DDLDREDDIR LLKYLFTLIR AGMTDEAQRL CKRCGQAWRA ATLEGWKLYH DANINGGTEL QAVEGNPYRC VWKT CCWRM AEDEQFNKYE RAIYATLSGN LKQLLPVCES WEDTVWAHFK VMVDSLVEQE IRASIISFNE ANELPREYLE ANWTL DSVF EELQATDKKR VLEENREHYH IIQKFVILAD VDGLMDEFSE WLSNGKNLLL GHLLRFMTHL LLFFRTLGLQ AKEEVS VEV LKTYIQRLIN EKQIELIAFY VSHLPQELAI SQYAVFLENI TDPDQRQRCL ELAKEAGLDV ASITKTVVEN TRKKDAG EF AHHDFAPALD SGTSEEDRAK IDVIDWLVFD PAQRAEALKQ SNAIMRKFLA SKKHEAAKEV FAKIPQDSIA EIYSQWEE Q AMDSALPAED DNAIREHLCI RAYLESHEAF NEWFKHINSP PQKPTLVGQA SFTEKVAHEH KEKKYEMDFG IWKGHLDAL TSDVKEKIYN VLLFVDGGWM VDVREDTEED PERSHQMVLL RRLCLPMMCF LLHTVLHNTK QYKDCLRLAD IVSSENQKLY TVFSKTEMR NLLQKLRESS LMLLDLQLDP LGYEIQS

UniProtKB: Nuclear pore complex protein

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Macromolecule #11: Nuclear pore complex protein Nup93

MacromoleculeName: Nuclear pore complex protein Nup93 / type: protein_or_peptide / ID: 11 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Xenopus laevis (African clawed frog)
Molecular weightTheoretical: 93.565156 KDa
SequenceString: MDGEGFGELL QQAEQLAAET EGVTELPHVE RNLQEIQQAG ERLRSKTMTR TSQESANVKA SVLLGSRGLD ISHISQRLES LSAATTFEP LEPVKDTDIQ GFLKNEKDNA LLSAIEESRK RTFVMAEEYH RESMLVEWEQ VKQRVLHTLL ASGEDALDFT Q ESETSYIS ...String:
MDGEGFGELL QQAEQLAAET EGVTELPHVE RNLQEIQQAG ERLRSKTMTR TSQESANVKA SVLLGSRGLD ISHISQRLES LSAATTFEP LEPVKDTDIQ GFLKNEKDNA LLSAIEESRK RTFVMAEEYH RESMLVEWEQ VKQRVLHTLL ASGEDALDFT Q ESETSYIS ESGAPGRSSL DNVEMAYARQ MYMYNEKVVS GHLQPSLVDL CTEAAERLDD KNVSDLWVMV KQMTDVPLIP AS DTLKSRC SGQMQMAFVR QALNYLEQSY KNYTLISVFA NLQQAQLGGV PGTYNLVRSF LNIRLPTPIP GLQDGEIEGY PVW ALIYYC MRCGDLMAAQ QVVNRAQHQL GDFKNCFQEY IHNKDRRLSP TTENKLRLHY RRAVRASTDP YKRAVYCIIG RCDV SDNHS EVADKTEDYL WLKLSQVCFE DEANSSPQDR LTLPQFQKQL FEDYGESHFA VNQQPYLYFQ VLFLTAQFEA AIAFL FRLE RTRCHAVHVA LALFELKLLL KSTGQSAQLL SQEPGEPQGV RRLNFIRLLM LYTRKFEPTD PREALQYFYF LRNEKD NQG ESMFLRCVSE LVIESREFDM LLGKLEKDGS RKPGAIDKFT RDTKTIINKV ASVAENKGLF EEAAKLYDLA KNPDKVL EL TNKLLSPVVS QISAPQSNRE RLKNMALAIA ERYKSQGVSA EKSINSTFYL LLDLITFFDE YHAGHIDLSF DVIERLKL V PLSQDSVEER VAAFRNFSDE IRHNLSEILL ATMNILFTQY KRLKGSGPTT LGRPQRVQED KDSVLRSQAR ALITFAGMI PYRMSGDTNA RLVQMEVLMN

UniProtKB: Nuclear pore complex protein Nup93

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Macromolecule #12: MGC83295 protein

MacromoleculeName: MGC83295 protein / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xenopus laevis (African clawed frog)
Molecular weightTheoretical: 227.854141 KDa
SequenceString: MAAQLALNSE ASLWGPYREI WQTVLSALIK RQPEAVHSLD IVLKKYKPDF ISLFKNPPKS AQQHERVQKA STEGIPIKGT QRTRILEEQ LIKEAFILSD LYNIGEIAAV ELLLIGEQQQ PTFHGLTRGL VAILLYWDGK SCMAESLLHL IQARKGKTFT L DHSPEVVS ...String:
MAAQLALNSE ASLWGPYREI WQTVLSALIK RQPEAVHSLD IVLKKYKPDF ISLFKNPPKS AQQHERVQKA STEGIPIKGT QRTRILEEQ LIKEAFILSD LYNIGEIAAV ELLLIGEQQQ PTFHGLTRGL VAILLYWDGK SCMAESLLHL IQARKGKTFT L DHSPEVVS MVTRFTDDLM EQGLTNKILT LISQIDVNNE FDKLKKERGL GNKKHRKEVS DLIKECQQSL AHSLYSWSCQ TP LNREDTL LLIGYLEKVT VEGDGSLDKV NLTLLMSLLY CLDVGFLEQG TDDREELMKQ ASMFMDRQYI AAIHNRLQNT QPW KSPGMQ ATVRLAWALA LRGISQFSEV LEFSEADEPM AEIAIGGNVF LFLTEAVVGS ESFCTDEFFI RRIHKLVTDF PTLM PMKVK QLRNRAEEDA RLIQMSMQMG NEPPASLRRD LEHLLLLIGE LYRKDPFHLE LALEYWCPTE PLQSTSLMGS FLGVA HQRP PQRQVLLSKF VRQMSDLLPA TLYLPYLKML RGLASGPQCA HYCFSLLKAN GGSSAENLQA AGGSPVSWDH FFHSLM LYH EHLRRDLPNT DNIHQRHPPL RGITQRELDG LIACLQLTCT IIDWSESARL ALCEHAQWMP VVVILGLLQC SIPPLLK AE LLKTLAAFGK SPEIAASLWQ SLEYTQILQT VRATGLRQGV GIEVELNEIE SRCEEYPLTR AFCQLISTLV ESSFPTNL G AGLRAPGFEP YLQFLRDTVF LRYRTRAYRR AAEKWEVAEA VLDVFYKLLK DYEPQPEDFV DQYVELQGEE RVAFKPPGF SLMHHLLNES PMLELCLSLM EEGVTQLDTY APFPGKKHLE KAVAYCFMLL NLTLQKENRF MDLLRESHLS MIVTPLEQLL QGINPRSKK ADNVVNIARY LCHGNSNAEL AFESAKILCS ISCNSKIQEK IVGDFTQDQN VSQKLMVGFV SCLDSEEAEE L LDSEKEAE DQVKQTNIRY MTKIHILNLL ITSLEMKAPN LAMFLLGYEL KKPVSTTNLQ DSGVLGCPRT CLHSILDILR KG TDVRAGP VAVWDTPHLA ELCYQVIYQL CACADTSGPT MRYLRTSQDF LFSQLQHLPF SVEESEISAM NQMSWLMKTA TIE LRITSL NRQRSHTQRL LHLLLDDMPT RPYSADGEGG MEDESRSLSG FLHFDTTSKV RRKILRILDS IQFSNEIPEP LQLD FFDRS QIEQVIANCE HKNRRGQTVC NVKLLHRVLV AEVNALQGMA AIGQRPLLME EINTILQYVV ERNKLLQCLH AKRHA LESW RQLVEIILTA CPQDLIPTEH RQLIIRDLLQ DLHVKILDDD AAQELMPIVA GAVFTLTAHL SQSVRTELKQ PMTASG LGQ SQYVQMLDGS FAAPPGTENI SAGFASIGDS SLHMILRNLL EFILKTGGGF QRVRAHLYGS LLYYLQIAQR PDEPDTL ES AHKSMWERLT APEDVFSKLQ RDNLSIFESY GTALMEVVCR DACDGHDIGR MLALALLDRI VSVDRQQQWL LYLSNSGY L KVLVDSLAED DVVLRNLLTP QPPLLKALYI YESKMAFLTR VAKSSQGAIE LLRSGVIVRL AQCQVYDMRP ETDPHGVFG MRETPVFIPA PVERYRQILL PALQICQLIL TSSTAQHLQA AGQVLQFLVA HSDTIQAILR SQEGSLGSLQ ELALLTGIIS KAALPGVLN ELDIGLNDGS MMELQGHIGR FQRQCLALLN RFGGSDRLRQ LSLQDDSSRL DGVSKKDDME LAMQQICSNV M EYCQALMI QNSPSFQQTV CLFTPSLKES ASRDGTRQDS QVSILPSWRL PSLGVVIHLL KQSANNFFTY YDIHRQSVGK LQ NVEQLPP DEIKELCQSE MPVGADKIST TQKYGLARRR LVKLINSRAK LLSLCSYIIE TCLYILWRHL EYYLLHCTTS DSQ DPVFSN MTFGNRRFQD TFNTDPNMDP RNLRQNKVSQ QDVDTLLREG ANSFGESLQK RLLDIESLYC KVRSRHSFIQ ALVR RIRGL LRVSRV

UniProtKB: MGC83295 protein

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Macromolecule #13: Protein ELYS

MacromoleculeName: Protein ELYS / type: protein_or_peptide / ID: 13 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Xenopus laevis (African clawed frog)
Molecular weightTheoretical: 268.771125 KDa
SequenceString: MQNLEAQVTG SLVAFPDVTQ KALKEDEINL DSVLRGKFST GRTSLAWLAC GPQLEITNSV TGERISAYHF SGLTERPPVV VAVKEFTWQ KKTGLLVGLV EAEGSVLCLY DIGISKVVKA VVLPGSVTAV EPIINHGGAS ASTQHLHQSL RWFFGVTAVV T DVGHVLLI ...String:
MQNLEAQVTG SLVAFPDVTQ KALKEDEINL DSVLRGKFST GRTSLAWLAC GPQLEITNSV TGERISAYHF SGLTERPPVV VAVKEFTWQ KKTGLLVGLV EAEGSVLCLY DIGISKVVKA VVLPGSVTAV EPIINHGGAS ASTQHLHQSL RWFFGVTAVV T DVGHVLLI DLCLDEVSSN QDELDASDLE VMSVIPTKIP KLREAATRER RHLCLQLAAP TGTTVSCLSY ISRTNQLAVG YS DGYFSLW NMKTLRRDYH VQIEGGRVPV CAVAFQEPEN DPRNCCYLWA VQSSESGGDV SLHLLQLAFS DRKCLASGQI MYE LLEYCE ERYSLDLSGS TLSLRGQSNN TKLLGCQTIE KFRVHGERED GVHEVTSPDT SVSVFSWQVN TYGQGKPSVY LGVF DINRW YQAQMPDSLR SGQFLRNCSY FAFWSLEAVV NITTQDIIFD ILVHERSLSR GIPPSYPPPE QFYYPSTYNF DATCL LNSG LIHFACTGFQ KETLHFLKKS GSSLNEAIPD GYNRCLAAGL LAPKFTDVQA SSLSQEEQLQ AILAAAVETS SLGLLT SCI KRWTAEEQPR SAANLRFVLE WTWKKVTLTK QEFDRLCFRL FDGSCNFIDP HTLQSLQQCH LYFSNLTAVL NCFIAQA KE VTQQGAVDLT NKQSVTRLLT LYASVVLWFC RSGMLPDSSD ETVQLTRPFY NYQVIQQYYS DQRKKLERLA RGKWDTSS L MIDGLINQFG DRIQQLWSRD DNGTGKYPPA NLHALLDVYL LENADEMSKH AITIYFLLDI MYSFPDKPDS SIESFPTAF FVPGSLIKLI QGFWLLDHND YQNSVDCILN PASSRVMSWQ HSQIIENLLC HGDSRQALRY LQVMKPVATT SKEVKLHMTV LLANRSILE AWNLQRLHSS RLNVEELLKH MYEMCQEMGL IEELLKLTFT DFEQGYLHKF LQTTGVQNQE LLLVHHLQRA N YISALQLN QSLKTNHLND CDRRLRERSG ARNAILDQYG KILPRVQRTL ASERAKPYSL PSLVWREVAR PKPLSTTAKQ AA PGSIITK ANFICNVLSK IKEVSTANEK REEYSPYQSM VSEEPTAPPL QDIDVPDAFF GTPINKSRRV SRLLDSVVHP VLM EPTPLT SSDTDNNQTP HKSPLLKTSS PLHSSLRRIA HMRSFAKASE FSLLETPLVV RKAKALAANT ASSGYTSITP QSIL RSSVR TTPLVSPSVS PGRSLTPPLR PKETKISFME LSFTRHAKAA HSSEGNLLAI SPVLRSSPDA VWSVKGKVAS FTQNT PVKK LDEIDASSSG IQEESQDEME VSKEISNISV RSEQASLEYH DAPTPEDLEN DEISGTTNSQ PQVNEVHHQM EDGQLT EKP AELALTEMQE EFIDSEEREI EYISAPLNGP NALECMTAVP DIYLEDASQC ILETPEGSSV SVTGEQECVS SAKDSES VI SIHDSDDAHS NLSENDQDSE EIEENNLRVP TTVTRCEEFD LIETKDLEVE LEEADSEKTN YKDIYPDATV QLGFTVES I EQRYTCELAD RRETPSETDE IEGEHFETEN NFSLVLEGDV TEEEILEPSS SKTDLELTRP PIAHQKLISE NRENIENCE TTEKIPANMS PLVDSDHESK TLETLPSEAD LSVAEKVLKG TEEKDVPPEV HSEVVLESKL VGNAMMSLDS SESQEVIISQ YDNVISIEK LEMTQEKMYG EKTEQINEGQ VSPNRDQSTL VKPLTPRRSI RKSSKPADSS TDIIGNITLP TTPKRGLKKA K ENVDTLKN SISVVPEEEL TLGTRRITRK ATLTALDNPE PLQIKEPPSG EDLQVQPSTP TRGRRGKVIT SDDLKEPPSG ED LQVQPST PTRGRRGRVI TSDDLREPPP GEDLQVQPST PTRGRRGRVI TSDDIKESPS VEDLQVQPST PTRGRRGKVI TSD DIKEPP SVEDLQVQPS TPTRGRKGKV ITSDDIKEPL SGEDLQVQPS TPTRGRKGKV ITSDDIKEPL SEEVLQEQPS TPTR GRRGR VITSDGKGYE CVEEKNALPL TPTRITRSKN ILEPEKGISQ IEPEKGISQI EPDKGLSQIE DTGETEHEVV TPRRG RRGK RVVNELVKHF ERNSSQPNIK ADTSPPVSPK KVSLRWTRTR SENQRINATE EQASKIQEDL SDTPRKRYKK SSNKMG FEE TTDTVTEGAI VEDVQESLII SHLGKNPNTS IVRSARKTAL PPVTEDHSEQ PLLPPESHSK VHSSLAIADE ENKTNTR TR SGNKSSVDVS AITFEFSTPK ARTKKTAKGS AVPTELIPST QYVFSPPSTR TRRATRANVS EAVIEPQLQF QESCEIAE T EVPEVPASKP RGRPPKHKAK AVTRVLKKPS WSTPPVEIKL ISPPESPAVS ETNTKTDSTE AKGAEKISVR RTRRRIIAK PVTRRKMR

UniProtKB: Protein ELYS

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 4.0 µm / Nominal defocus min: 1.2 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 5.6 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 813020
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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