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Structure paper

TitleFunctional and Structural Dissection of a Plant Steroid 3-O-Glycosyltransferase Facilitated the Engineering Enhancement of Sugar Donor Promiscuity
Journal, issue, pagesAcs Catalysis, Page 2927-2937, Year 2022
Publish dateNov 18, 2021 (structure data deposition date)
AuthorsHuang, W. / He, Y. / Jiang, R. / Deng, Z. / Long, F.
External linksAcs Catalysis / Search PubMed
MethodsX-ray diffraction
Resolution1.95 - 2.45 Å
Structure data

PDB-7w09:
UGT74AN2, Plant Steroid Glycosyltransferase
Method: X-RAY DIFFRACTION / Resolution: 1.95 Å

PDB-7w0k:
plant glycosyltransferase
Method: X-RAY DIFFRACTION / Resolution: 2.04 Å

PDB-7w0z:
Glycosyltranferase UGT74AN2
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-7w10:
UGT74AN2
Method: X-RAY DIFFRACTION / Resolution: 2.15 Å

PDB-7w11:
glycosyltransferase
Method: X-RAY DIFFRACTION / Resolution: 2.45 Å

PDB-7w1b:
Glycosyltransferase
Method: X-RAY DIFFRACTION / Resolution: 2.15 Å

PDB-7w1h:
glycosyltransferase
Method: X-RAY DIFFRACTION / Resolution: 2.15 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-UDP:
URIDINE-5'-DIPHOSPHATE / UDP*YM

ChemComp-GOL:
GLYCEROL

ChemComp-6JI:
5-[(1R,2S,4R,6R,7R,10S,11S,14S,16R)-14-hydroxy-7,11-dimethyl-3-oxapentacyclo[8.8.0.02,4.02,7.011,16]octadecan-6-yl]pyran-2-one

ChemComp-TRS:
2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH buffer*YM

ChemComp-BUF:
bufalin / toxin*YM

ChemComp-GLC:
alpha-D-glucopyranose

ChemComp-DTX:
DIGITOXIGENIN / toxin*YM

ChemComp-UPG:
URIDINE-5'-DIPHOSPHATE-GLUCOSE

Source
  • calotropis gigantea (mudar)
KeywordsTRANSFERASE / Apo / UGT74AN2 / Glycosyltransferase / UGT74AN2 Complex with UDP / Complex with resibufagenin / Complex / COMPLEX WITH UDPG

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