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TitleStructural and mechanistic basis for recognition of alternative tRNA precursor substrates by bacterial ribonuclease P.
Journal, issue, pagesNat Commun, Vol. 13, Issue 1, Page 5120, Year 2022
Publish dateAug 31, 2022
AuthorsJiaqiang Zhu / Wei Huang / Jing Zhao / Loc Huynh / Derek J Taylor / Michael E Harris /
PubMed AbstractBinding of precursor tRNAs (ptRNAs) by bacterial ribonuclease P (RNase P) involves an encounter complex (ES) that isomerizes to a catalytic conformation (ES*). However, the structures of ...Binding of precursor tRNAs (ptRNAs) by bacterial ribonuclease P (RNase P) involves an encounter complex (ES) that isomerizes to a catalytic conformation (ES*). However, the structures of intermediates and the conformational changes that occur during binding are poorly understood. Here, we show that pairing between the 5' leader and 3'RCCA extending the acceptor stem of ptRNA inhibits ES* formation. Cryo-electron microscopy single particle analysis reveals a dynamic enzyme that becomes ordered upon formation of ES* in which extended acceptor stem pairing is unwound. Comparisons of structures with alternative ptRNAs reveals that once unwinding is completed RNase P primarily uses stacking interactions and shape complementarity to accommodate alternative sequences at its cleavage site. Our study reveals active site interactions and conformational changes that drive molecular recognition by RNase P and lays the foundation for understanding how binding interactions are linked to helix unwinding and catalysis.
External linksNat Commun / PubMed:36045135 / PubMed Central
MethodsEM (single particle)
Resolution3.1 - 3.4 Å
Structure data

26636

7uo0

EMDB-26636, PDB-7uo0:
E.coli RNaseP Holoenzyme with Mg2+
Method: EM (single particle) / Resolution: 3.4 Å

26637

7uo1

EMDB-26637, PDB-7uo1:
E.coli RNaseP Holoenzyme with Mg2+
Method: EM (single particle) / Resolution: 3.2 Å

26638

7uo2

EMDB-26638, PDB-7uo2:
E.coli RNaseP Holoenzyme with Mg2+
Method: EM (single particle) / Resolution: 3.1 Å

26640

7uo5

EMDB-26640, PDB-7uo5:
E.coli RNaseP Holoenzyme with Mg2+
Method: EM (single particle) / Resolution: 3.1 Å

Chemicals

ChemComp-CA:
Unknown entry

ChemComp-MG:
Unknown entry

Source
  • escherichia coli (E. coli)
KeywordsHYDROLASE/RNA / ribozyme / protein-RNA complex / divalent ion / RNA / HYDROLASE-RNA complex

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