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TitleStructures of a mobile intron retroelement poised to attack its structured DNA target.
Journal, issue, pagesScience, Vol. 378, Issue 6620, Page 627-634, Year 2022
Publish dateNov 11, 2022
AuthorsKevin Chung / Ling Xu / Pengxin Chai / Junhui Peng / Swapnil C Devarkar / Anna Marie Pyle /
PubMed AbstractGroup II introns are ribozymes that catalyze their self-excision and function as retroelements that invade DNA. As retrotransposons, group II introns form ribonucleoprotein (RNP) complexes that roam ...Group II introns are ribozymes that catalyze their self-excision and function as retroelements that invade DNA. As retrotransposons, group II introns form ribonucleoprotein (RNP) complexes that roam the genome, integrating by reversal of forward splicing. Here we show that retrotransposition is achieved by a tertiary complex between a structurally elaborate ribozyme, its protein mobility factor, and a structured DNA substrate. We solved cryo-electron microscopy structures of an intact group IIC intron-maturase retroelement that was poised for integration into a DNA stem-loop motif. By visualizing the RNP before and after DNA targeting, we show that it is primed for attack and fits perfectly with its DNA target. This study reveals design principles of a prototypical retroelement and reinforces the hypothesis that group II introns are ancient elements of genetic diversification.
External linksScience / PubMed:36356138 / PubMed Central
MethodsEM (single particle)
Resolution2.8 - 3.1 Å
Structure data

26549

7uim

EMDB-26549, PDB-7uim:
CryoEM Structure of an Group II Intron Retroelement (apo-complex)
Method: EM (single particle) / Resolution: 3.1 Å

26550

7uin

EMDB-26550, PDB-7uin:
CryoEM Structure of an Group II Intron Retroelement
Method: EM (single particle) / Resolution: 2.8 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-NH4:
AMMONIUM ION

ChemComp-HOH:
WATER

Source
  • [eubacterium] rectale (bacteria)
KeywordsRNA BINDING PROTEIN/RNA / RNA / intron / group II / maturase / splicing / retrotransposition / RNA BINDING PROTEIN-RNA complex / RNA BINDING PROTEIN/RNA/DNA / RNA BINDING PROTEIN-RNA-DNA complex

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