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TitleCryo-Electron Microscopy Reveals Cardiac Myosin Binding Protein-C M-Domain Interactions with the Thin Filament.
Journal, issue, pagesJ Mol Biol, Vol. 434, Issue 24, Page 167879, Year 2022
Publish dateNov 10, 2022
AuthorsCristina M Risi / Edwin Villanueva / Betty Belknap / Rachel L Sadler / Samantha P Harris / Howard D White / Vitold E Galkin /
PubMed AbstractCardiac myosin binding protein C (cMyBP-C) modulates cardiac contraction via direct interactions with cardiac thick (myosin) and thin (actin) filaments (cTFs). While its C-terminal domains (e.g. C8- ...Cardiac myosin binding protein C (cMyBP-C) modulates cardiac contraction via direct interactions with cardiac thick (myosin) and thin (actin) filaments (cTFs). While its C-terminal domains (e.g. C8-C10) anchor cMyBP-C to the backbone of the thick filament, its N-terminal domains (NTDs) (e.g. C0, C1, M, and C2) bind to both myosin and actin to accomplish its dual roles of inhibiting thick filaments and activating cTFs. While the positions of C0, C1 and C2 on cTF have been reported, the binding site of the M-domain on the surface of the cTF is unknown. Here, we used cryo-EM to reveal that the M-domain interacts with actin via helix 3 of its ordered tri-helix bundle region, while the unstructured part of the M-domain does not maintain extensive interactions with actin. We combined the recently obtained structure of the cTF with the positions of all the four NTDs on its surface to propose a complete model of the NTD binding to the cTF. The model predicts that the interactions of the NTDs with the cTF depend on the activation state of the cTF. At the peak of systole, when bound to the extensively activated cTF, NTDs would inhibit actomyosin interactions. In contrast, at falling Ca levels, NTDs would not compete with the myosin heads for binding to the cTF, but would rather promote formation of active cross-bridges at the adjacent regulatory units located at the opposite cTF strand. Our structural data provides a testable model of the cTF regulation by the cMyBP-C.
External linksJ Mol Biol / PubMed:36370805 / PubMed Central
MethodsEM (helical sym.)
Resolution8.0 Å
Structure data

25911

7tij

EMDB-25911, PDB-7tij:
Cardiac F-actin decorated with regulatory M-domain of cardiac myosin binding protein C
Method: EM (helical sym.) / Resolution: 8.0 Å

25914

7tit

EMDB-25914, PDB-7tit:
Cardiac thin filament decorated with regulatory M-domain of cardiac myosin binding protein C
Method: EM (helical sym.) / Resolution: 8.0 Å

25918

7tj7

EMDB-25918, PDB-7tj7:
Cardiac thin filament decorated with C1 Ig-domain and regulatory M-domain of cardiac myosin binding protein C (cMyBP-C)
Method: EM (helical sym.) / Resolution: 8.0 Å

Source
  • pig (pig)
  • homo sapiens (human)
  • sus scrofa (pig)
KeywordsMOTOR PROTEIN / cardiac contraction regulator / muscle protein / muscle contraction regulator

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