Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Architecture and self-assembly of the jumbo bacteriophage nuclear shell.
Journal, issue, pages	Nature, Vol. 608, Issue 7922, Page 429-435, Year 2022
Publish date	Aug 3, 2022
Authors	Thomas G Laughlin / Amar Deep / Amy M Prichard / Christian Seitz / Yajie Gu / Eray Enustun / Sergey Suslov / Kanika Khanna / Erica A Birkholz / Emily Armbruster / J Andrew McCammon / Rommie E Amaro / Joe Pogliano / Kevin D Corbett / Elizabeth Villa /
PubMed Abstract	Bacteria encode myriad defences that target the genomes of infecting bacteriophage, including restriction-modification and CRISPR-Cas systems. In response, one family of large bacteriophages uses a ...Bacteria encode myriad defences that target the genomes of infecting bacteriophage, including restriction-modification and CRISPR-Cas systems. In response, one family of large bacteriophages uses a nucleus-like compartment to protect its replicating genomes by excluding host defence factors. However, the principal composition and structure of this compartment remain unknown. Here we find that the bacteriophage nuclear shell assembles primarily from one protein, which we name chimallin (ChmA). Combining cryo-electron tomography of nuclear shells in bacteriophage-infected cells and cryo-electron microscopy of a minimal chimallin compartment in vitro, we show that chimallin self-assembles as a flexible sheet into closed micrometre-scale compartments. The architecture and assembly dynamics of the chimallin shell suggest mechanisms for its nucleation and growth, and its role as a scaffold for phage-encoded factors mediating macromolecular transport, cytoskeletal interactions, and viral maturation.
External links	Nature / PubMed:35922510 / PubMed Central
Methods	EM (subtomogram averaging) / EM (single particle)
Resolution	2.3 - 27.0 Å
Structure data	EMDB-25183: P. chlororaphis 70S ribosome in situ subtomogram average Method: EM (subtomogram averaging) / Resolution: 10.2 Å EMDB-25220: In situ subtomogram average of the 201phi2-1 phage nucleus major shell protein, chimallin (concave class) Method: EM (subtomogram averaging) / Resolution: 20.0 Å EMDB-25221: In situ consensus subtomogram average of the 201phi2-1 chimallin Method: EM (subtomogram averaging) / Resolution: 24.0 Å EMDB-25222: In situ subtomogram average of 201phi2-1 phage nucleus major shell protein, chimallin (intermediate/flat class) Method: EM (subtomogram averaging) / Resolution: 18.0 Å EMDB-25223: In situ subtomogram average of the 201phi2-1 phage nucleus major shell protein, chimallin (convex class) Method: EM (subtomogram averaging) / Resolution: 23.0 Å EMDB-25229: In situ subtomogram average of the Goslar major phage nucleus shell protein, chimallin (consensus class) Method: EM (subtomogram averaging) / Resolution: 27.0 Å EMDB-25262: In situ subtomogram average of Goslar phage nucleus major shell protein, chimallin (concave class) Method: EM (subtomogram averaging) / Resolution: 20.0 Å EMDB-25358: In situ subtomogram average of the major Goslar phage nucleus shell protein, chimallin (convex class) Method: EM (subtomogram averaging) / Resolution: 25.0 Å EMDB-25359: In situ subtomogram average of the APEC2248 70S ribosome Method: EM (subtomogram averaging) / Resolution: 8.53 Å EMDB-25360: In situ subtomogram average of the APEC2248 50S ribosome Method: EM (subtomogram averaging) / Resolution: 12.0 Å 25390 7sqq EMDB-25390, PDB-7sqq: 201Phi2-1 Chimallin Cubic (O, 24mer) assembly Method: EM (single particle) / Resolution: 4.2 Å 25391 7sqr EMDB-25391, PDB-7sqr: 201phi2-1 Chimallin localized tetramer reconstruction Method: EM (single particle) / Resolution: 3.4 Å 25392 7sqs EMDB-25392, PDB-7sqs: 201phi2-1 Chimallin C1 localized reconstruction Method: EM (single particle) / Resolution: 3.1 Å EMDB-25393: 201phi2-1 chimallin rectangular (D4,40mer) assembly Method: EM (single particle) / Resolution: 21.0 Å 25394 7sqt EMDB-25394, PDB-7sqt: Goslar chimallin cubic (O, 24mer) assembly Method: EM (single particle) / Resolution: 4.0 Å 25395 7squ EMDB-25395, PDB-7squ: Goslar chimallin C4 tetramer localized reconstruction Method: EM (single particle) / Resolution: 2.6 Å 25396 7sqv EMDB-25396, PDB-7sqv: Goslar chimallin C1 localized reconstruction Method: EM (single particle) / Resolution: 2.3 Å
Chemicals	ChemComp-HOH: WATER
Source	Pseudomonas chlororaphis (bacteria) pseudomonas phage 201phi2-1 (virus) Goslarvirus Escherichia coli (E. coli) escherichia phage vb_ecom_goslar (virus)
Keywords	STRUCTURAL PROTEIN / Phage / virus / viral protein