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TitleThe human SKI complex regulates channeling of ribosome-bound RNA to the exosome via an intrinsic gatekeeping mechanism.
Journal, issue, pagesMol Cell, Vol. 82, Issue 4, Page 756-769.e8, Year 2022
Publish dateFeb 17, 2022
AuthorsAlexander Kögel / Achim Keidel / Fabien Bonneau / Ingmar B Schäfer / Elena Conti /
PubMed AbstractThe superkiller (SKI) complex is the cytoplasmic co-factor and regulator of the RNA-degrading exosome. In human cells, the SKI complex functions mainly in co-translational surveillance-decay ...The superkiller (SKI) complex is the cytoplasmic co-factor and regulator of the RNA-degrading exosome. In human cells, the SKI complex functions mainly in co-translational surveillance-decay pathways, and its malfunction is linked to a severe congenital disorder, the trichohepatoenteric syndrome. To obtain insights into the molecular mechanisms regulating the human SKI (hSKI) complex, we structurally characterized several of its functional states in the context of 80S ribosomes and substrate RNA. In a prehydrolytic ATP form, the hSKI complex exhibits a closed conformation with an inherent gating system that effectively traps the 80S-bound RNA into the hSKI2 helicase subunit. When active, hSKI switches to an open conformation in which the gating is released and the RNA 3' end exits the helicase. The emerging picture is that the gatekeeping mechanism and architectural remodeling of hSKI underpin a regulated RNA channeling system that is mechanistically conserved among the cytoplasmic and nuclear helicase-exosome complexes.
External linksMol Cell / PubMed:35120588 / PubMed Central
MethodsEM (single particle)
Resolution3.1 - 6.5 Å
Structure data

13923

7qdr

EMDB-13923, PDB-7qdr:
Apo human SKI complex in the closed state
Method: EM (single particle) / Resolution: 3.7 Å

13925

7qds

EMDB-13925, PDB-7qds:
Apo human SKI complex in the open state
Method: EM (single particle) / Resolution: 3.8 Å

13927

7qdy

EMDB-13927, PDB-7qdy:
RNA-bound human SKI complex
Method: EM (single particle) / Resolution: 3.1 Å

13928

7qdz

EMDB-13928, PDB-7qdz:
80S-bound human SKI complex in the closed state
Method: EM (single particle) / Resolution: 3.6 Å

13929

7qe0

EMDB-13929, PDB-7qe0:
80S-bound human SKI complex in the open state
Method: EM (single particle) / Resolution: 6.5 Å

Source
  • homo sapiens (human)
KeywordsRNA BINDING PROTEIN / multiprotein complex / RNA helicase / DExH-box helicase / ATPase / RNA binding

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