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TitleStructural basis of sodium-dependent bile salt uptake into the liver.
Journal, issue, pagesNature, Vol. 606, Issue 7916, Page 1015-1020, Year 2022
Publish dateMay 11, 2022
AuthorsKapil Goutam / Francesco S Ielasi / Els Pardon / Jan Steyaert / Nicolas Reyes /
PubMed AbstractThe liver takes up bile salts from blood to generate bile, enabling absorption of lipophilic nutrients and excretion of metabolites and drugs. Human Na-taurocholate co-transporting polypeptide (NTCP) ...The liver takes up bile salts from blood to generate bile, enabling absorption of lipophilic nutrients and excretion of metabolites and drugs. Human Na-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. NTCP is also the cellular entry receptor of human hepatitis B and D viruses (HBV/HDV), and has emerged as an important target for antiviral drugs. However, the molecular mechanisms underlying NTCP transport and viral receptor functions remain incompletely understood. Here we present cryo-electron microscopy structures of human NTCP in complexes with nanobodies, revealing key conformations of its transport cycle. NTCP undergoes a conformational transition opening a wide transmembrane pore that serves as the transport pathway for bile salts, and exposes key determinant residues for HBV/HDV binding to the outside of the cell. A nanobody that stabilizes pore closure and inward-facing states impairs recognition of the HBV/HDV receptor-binding domain preS1, demonstrating binding selectivity of the viruses for open-to-outside over inward-facing conformations of the NTCP transport cycle. These results provide molecular insights into NTCP 'gated-pore' transport and HBV/HDV receptor recognition mechanisms, and are expected to help with development of liver disease therapies targeting NTCP.
External linksNature / PubMed:35545671 / PubMed Central
MethodsEM (single particle)
Resolution3.3 - 3.7 Å
Structure data

13593

7pqg

EMDB-13593, PDB-7pqg:
Structure of thermostabilised human NTCP in complex with nanobody 87
Method: EM (single particle) / Resolution: 3.7 Å

13596

7pqq

EMDB-13596, PDB-7pqq:
Structure of thermostabilised human NTCP in complex with Megabody 91
Method: EM (single particle) / Resolution: 3.3 Å

Source
  • lama glama (llama)
  • homo sapiens (human)
KeywordsMEMBRANE PROTEIN / SLC10A1 / sodium bile acid symporter / bile acid transporter

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