Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structure of Human Enterovirus 70 and Its Inhibition by Capsid-Binding Compounds.
Journal, issue, pages	J Virol, Vol. 96, Issue 17, Page e0060422, Year 2022
Publish date	Aug 8, 2022
Authors	Tibor Füzik / Jana Moravcová / Sergei Kalynych / Pavel Plevka /
PubMed Abstract	Enterovirus 70 (EV70) is a human pathogen belonging to the family . EV70 is transmitted by eye secretions and causes acute hemorrhagic conjunctivitis, a serious eye disease. Despite the severity of ...Enterovirus 70 (EV70) is a human pathogen belonging to the family . EV70 is transmitted by eye secretions and causes acute hemorrhagic conjunctivitis, a serious eye disease. Despite the severity of the disease caused by EV70, its structure is unknown. Here, we present the structures of the EV70 virion, altered particle, and empty capsid determined by cryo-electron microscopy. The capsid of EV70 is composed of the subunits VP1, VP2, VP3, and VP4. The partially collapsed hydrophobic pocket located in VP1 of the EV70 virion is not occupied by a pocket factor, which is commonly present in other enteroviruses. Nevertheless, we show that the pocket can be targeted by the antiviral compounds WIN51711 and pleconaril, which block virus infection. The inhibitors prevent genome release by stabilizing EV70 particles. Knowledge of the structures of complexes of EV70 with inhibitors will enable the development of capsid-binding therapeutics against this virus. Globally distributed enterovirus 70 (EV70) causes local outbreaks of acute hemorrhagic conjunctivitis. The discharge from infected eyes enables the high-efficiency transmission of EV70 in overcrowded areas with low hygienic standards. Currently, only symptomatic treatments are available. We determined the structures of EV70 in its native form, the genome release intermediate, and the empty capsid resulting from genome release. Furthermore, we elucidated the structures of EV70 in complex with two inhibitors that block virus infection, and we describe the mechanism of their binding to the virus capsid. These results enable the development of therapeutics against EV70.
External links	J Virol / PubMed:35939401 / PubMed Central
Methods	EM (single particle)
Resolution	2.31 - 4.29 Å
Structure data	13022 7opx EMDB-13022, PDB-7opx: CryoEM structure of human enterovirus 70 native virion Method: EM (single particle) / Resolution: 2.63 Å 13125 7ozi EMDB-13125, PDB-7ozi: CryoEM structure of human enterovirus 70 A-particle Method: EM (single particle) / Resolution: 3.73 Å 13126 7ozj EMDB-13126, PDB-7ozj: CryoEM structure of human enterovirus 70 empty particle Method: EM (single particle) / Resolution: 4.29 Å 13127 7ozk EMDB-13127, PDB-7ozk: CryoEM structure of human enterovirus 70 in complex with Pleconaril Method: EM (single particle) / Resolution: 2.31 Å 13128 7ozl EMDB-13128, PDB-7ozl: CryoEM structure of human enterovirus 70 in complex with WIN51711 Method: EM (single particle) / Resolution: 2.74 Å
Chemicals	ChemComp-HOH: WATER / Water ChemComp-W11: 3-{3,5-DIMETHYL-4-[3-(3-METHYL-ISOXAZOL-5-YL)-PROPOXY]-PHENYL}-5-TRIFLUOROMETHYL-[1,2,4]OXADIAZOLE / antivirusYM / Pleconaril ChemComp-W71*: 5-(7-(4-(4,5-DIHYDRO-2-OXAZOLYL)PHENOXY)HEPTYL)-3-METHYL ISOXAZOLE
Source	EV70, EV-70 human enterovirus 70 (strain j670/71) human enterovirus 70
Keywords	VIRUS / EV70 / enterovirus 70 / virion / native / enterovirus / altered particle / human enterovirus / acute hemorrhagic conjunctivitis / empty particle / pleconaril / WIN51711 / accute hemorrhagic conjunctivitis