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TitleHigher-order phosphatase-substrate contacts terminate the integrated stress response.
Journal, issue, pagesNat Struct Mol Biol, Vol. 28, Issue 10, Page 835-846, Year 2021
Publish dateOct 8, 2021
AuthorsYahui Yan / Heather P Harding / David Ron /
PubMed AbstractMany regulatory PPP1R subunits join few catalytic PP1c subunits to mediate phosphoserine and phosphothreonine dephosphorylation in metazoans. Regulatory subunits engage the surface of PP1c, locally ...Many regulatory PPP1R subunits join few catalytic PP1c subunits to mediate phosphoserine and phosphothreonine dephosphorylation in metazoans. Regulatory subunits engage the surface of PP1c, locally affecting flexible access of the phosphopeptide to the active site. However, catalytic efficiency of holophosphatases towards their phosphoprotein substrates remains unexplained. Here we present a cryo-EM structure of the tripartite PP1c-PPP1R15A-G-actin holophosphatase that terminates signaling in the mammalian integrated stress response (ISR) in the pre-dephosphorylation complex with its substrate, translation initiation factor 2α (eIF2α). G-actin, whose essential role in eIF2α dephosphorylation is supported crystallographically, biochemically and genetically, aligns the catalytic and regulatory subunits, creating a composite surface that engages the N-terminal domain of eIF2α to position the distant phosphoserine-51 at the active site. Substrate residues that mediate affinity for the holophosphatase also make critical contacts with eIF2α kinases. Thus, a convergent process of higher-order substrate recognition specifies functionally antagonistic phosphorylation and dephosphorylation in the ISR.
External linksNat Struct Mol Biol / PubMed:34625748 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution2.55 - 3.96 Å
Structure data

12665

7nzm

EMDB-12665, PDB-7nzm:
Cryo-EM structure of pre-dephosphorylation complex of phosphorylated eIF2alpha with trapped holophosphatase (PP1A_D64A/PPP1R15A/G-actin/DNase I)
Method: EM (single particle) / Resolution: 3.96 Å

PDB-7nxv:
Crystal structure of the complex of DNase I/G-actin/PPP1R15A_582-621
Method: X-RAY DIFFRACTION / Resolution: 2.55 Å

Chemicals

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

ChemComp-CA:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-MN:
Unknown entry

Source
  • homo sapiens (human)
  • oryctolagus cuniculus (rabbit)
  • Rabbit (rabbit)
  • Bovine (cattle)
  • escherichia coli (strain k12) (bacteria)
  • bos taurus (cattle)
KeywordsHYDROLASE / hydrolase regulator / protein phosphatase 1 regulatory unit / holophosphatase / PP1 / PPP1R15A / phosphorylated eIF2alpha

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