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Structure paper

TitleStructural basis of translation termination, rescue, and recycling in mammalian mitochondria.
Journal, issue, pagesMol Cell, Vol. 81, Issue 12, Page 2566-22582.e6, Year 2021
Publish dateJun 17, 2021
AuthorsEva Kummer / Katharina Noel Schubert / Tanja Schoenhut / Alain Scaiola / Nenad Ban /
PubMed AbstractThe mitochondrial translation system originates from a bacterial ancestor but has substantially diverged in the course of evolution. Here, we use single-particle cryo-electron microscopy (cryo-EM) as ...The mitochondrial translation system originates from a bacterial ancestor but has substantially diverged in the course of evolution. Here, we use single-particle cryo-electron microscopy (cryo-EM) as a screening tool to identify mitochondrial translation termination mechanisms and to describe them in molecular detail. We show how mitochondrial release factor 1a releases the nascent chain from the ribosome when it encounters the canonical stop codons UAA and UAG. Furthermore, we define how the peptidyl-tRNA hydrolase ICT1 acts as a rescue factor on mitoribosomes that have stalled on truncated messages to recover them for protein synthesis. Finally, we present structural models detailing the process of mitochondrial ribosome recycling to explain how a dedicated elongation factor, mitochondrial EFG2 (mtEFG2), has specialized for cooperation with the mitochondrial ribosome recycling factor to dissociate the mitoribosomal subunits at the end of the translation process.
External linksMol Cell / PubMed:33878294
MethodsEM (single particle)
Resolution3.2 - 4.6 Å
Structure data

12527

7nqh

EMDB-12527, PDB-7nqh:
55S mammalian mitochondrial ribosome with mtRF1a and P-site tRNAMet
Method: EM (single particle) / Resolution: 3.5 Å

12529

7nql

EMDB-12529, PDB-7nql:
55S mammalian mitochondrial ribosome with ICT1 and P site tRNAMet
Method: EM (single particle) / Resolution: 3.4 Å

12567

7nsh

EMDB-12567, PDB-7nsh:
39S mammalian mitochondrial large ribosomal subunit with mtRRF (post) and mtEFG2
Method: EM (single particle) / Resolution: 3.2 Å

12568

7nsi

EMDB-12568, PDB-7nsi:
55S mammalian mitochondrial ribosome with mtRRF (pre) and tRNA(P/E)
Method: EM (single particle) / Resolution: 4.6 Å

12569

7nsj

EMDB-12569, PDB-7nsj:
55S mammalian mitochondrial ribosome with tRNA(P/P) and tRNA(E*)
Method: EM (single particle) / Resolution: 3.9 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

ChemComp-SPM:
SPERMINE

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

ChemComp-5GP:
GUANOSINE-5'-MONOPHOSPHATE

ChemComp-HOH:
WATER

ChemComp-GNP:
PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / GppNHp, GMPPNP, energy-carrying molecule analogue*YM

Source
  • sus scrofa (pig)
  • homo sapiens (human)
  • Pig (pig)
  • unidentified (others)
KeywordsTRANSLATION / Ribosome / Mitochondria / Termination / mtRF1a / Release / ICT1 / mtRRF / Recycling / tRNA(E*)

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