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Structure paper

TitleStructural mechanism for replication origin binding and remodeling by a metazoan origin recognition complex and its co-loader Cdc6.
Journal, issue, pagesNat Commun, Vol. 11, Issue 1, Page 4263, Year 2020
Publish dateAug 26, 2020
AuthorsJan Marten Schmidt / Franziska Bleichert /
PubMed AbstractEukaryotic DNA replication initiation relies on the origin recognition complex (ORC), a DNA-binding ATPase that loads the Mcm2-7 replicative helicase onto replication origins. Here, we report cryo- ...Eukaryotic DNA replication initiation relies on the origin recognition complex (ORC), a DNA-binding ATPase that loads the Mcm2-7 replicative helicase onto replication origins. Here, we report cryo-electron microscopy (cryo-EM) structures of DNA-bound Drosophila ORC with and without the co-loader Cdc6. These structures reveal that Orc1 and Orc4 constitute the primary DNA binding site in the ORC ring and cooperate with the winged-helix domains to stabilize DNA bending. A loop region near the catalytic Walker B motif of Orc1 directly contacts DNA, allosterically coupling DNA binding to ORC's ATPase site. Correlating structural and biochemical data show that DNA sequence modulates DNA binding and remodeling by ORC, and that DNA bending promotes Mcm2-7 loading in vitro. Together, these findings explain the distinct DNA sequence-dependencies of metazoan and S. cerevisiae initiators in origin recognition and support a model in which DNA geometry and bendability contribute to Mcm2-7 loading site selection in metazoans.
External linksNat Commun / PubMed:32848132 / PubMed Central
MethodsEM (single particle)
Resolution3.2 - 4.0 Å
Structure data

22329

7jgr

EMDB-22329, PDB-7jgr:
Structure of Drosophila ORC bound to DNA (84 bp) and Cdc6
Method: EM (single particle) / Resolution: 3.9 Å

22330

7jgs

EMDB-22330, PDB-7jgs:
Structure of Drosophila ORC bound to poly(dA/dT) DNA and Cdc6 (conformation 2)
Method: EM (single particle) / Resolution: 3.2 Å

22359

7jk2

EMDB-22359, PDB-7jk2:
Structure of Drosophila ORC bound to poly(dA/dT) DNA and Cdc6 (conformation 1)
Method: EM (single particle) / Resolution: 3.2 Å

22360

7jk3

EMDB-22360, PDB-7jk3:
Structure of Drosophila ORC bound to GC-rich DNA and Cdc6
Method: EM (single particle) / Resolution: 3.4 Å

22361

7jk4

EMDB-22361, PDB-7jk4:
Structure of Drosophila ORC bound to AT-rich DNA and Cdc6
Method: EM (single particle) / Resolution: 3.4 Å

22362

7jk5

EMDB-22362, PDB-7jk5:
Structure of Drosophila ORC bound to DNA
Method: EM (single particle) / Resolution: 3.9 Å

22363

7jk6

EMDB-22363, PDB-7jk6:
Structure of Drosophila ORC in the active conformation
Method: EM (single particle) / Resolution: 4.0 Å

Chemicals

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM / Adenosine triphosphate

ChemComp-MG:
Unknown entry

Source
  • drosophila melanogaster (fruit fly)
  • synthetic construct (others)
KeywordsREPLICATION / origin recognition complex / DNA replication initiation / DNA binding / REPLICATION/DNA / REPLICATION-DNA complex

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