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TitleCryo-EM Structure of the Photosynthetic LH1-RC Complex from .
Journal, issue, pagesBiochemistry, Year 2021
Publish dateJul 29, 2021
AuthorsKazutoshi Tani / Ryo Kanno / Xuan-Cheng Ji / Malgorzata Hall / Long-Jiang Yu / Yukihiro Kimura / Michael T Madigan / Akira Mizoguchi / Bruno M Humbel / Zheng-Yu Wang-Otomo /
PubMed Abstract () is one of the most widely used model organisms in bacterial photosynthesis. This purple phototroph is characterized by the presence of both rhodoquinone (RQ) and ubiquinone as electron carriers ... () is one of the most widely used model organisms in bacterial photosynthesis. This purple phototroph is characterized by the presence of both rhodoquinone (RQ) and ubiquinone as electron carriers and bacteriochlorophyll (BChl) esterified at the propionic acid side chain by geranylgeraniol (BChl ) instead of phytol. Despite intensive efforts, the structure of the light-harvesting-reaction center (LH1-RC) core complex from remains at low resolutions. Using cryo-EM, here we present a robust new view of the LH1-RC at 2.76 Å resolution. The LH1 complex forms a closed, slightly elliptical ring structure with 16 αβ-polypeptides surrounding the RC. Our biochemical analysis detected RQ molecules in the purified LH1-RC, and the cryo-EM density map specifically positions RQ at the Q site in the RC. The geranylgeraniol side chains of BChl coordinated by LH1 β-polypeptides exhibit a highly homologous tail-up conformation that allows for interactions with the bacteriochlorin rings of nearby LH1 α-associated BChls . The structure also revealed key protein-protein interactions in both N- and C-terminal regions of the LH1 αβ-polypeptides, mainly within a face-to-face structural subunit. Our high-resolution LH1-RC structure provides new insight for evaluating past experimental and computational results obtained with this old organism over many decades and lays the foundation for more detailed exploration of light-energy conversion, quinone transport, and structure-function relationships in this pigment-protein complex.
External linksBiochemistry / PubMed:34323477
MethodsEM (single particle)
Resolution2.76 Å
Structure data

31258

7eqd

EMDB-31258, PDB-7eqd:
STRUCTURE OF PHOTOSYNTHETIC LH1-RC SUPER-COMPLEX OF RHODOSPIRILLUM RUBRUM
Method: EM (single particle) / Resolution: 2.76 Å

Chemicals

ChemComp-07D:
Trans-Geranyl BACTERIOCHLOROPHYLL A

ChemComp-08I:
Trans-Geranyl BACTERIOPHEOPHYTIN A

ChemComp-U10:
UBIQUINONE-10

ChemComp-PGV:
(1R)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL (11E)-OCTADEC-11-ENOATE / phospholipid*YM

ChemComp-LMT:
DODECYL-BETA-D-MALTOSIDE / detergent*YM

ChemComp-FE:
Unknown entry

ChemComp-RQ0:
2-azanyl-5-[(2~{E},6~{E},8~{E},10~{E},12~{E},14~{E},18~{E},22~{E},26~{E},30~{E},34~{E})-3,7,11,15,19,23,27,31,35,39-decamethyltetraconta-2,6,8,10,12,14,18,22,26,30,34,38-dodecaenyl]-3-methoxy-6-methyl-cyclohexa-2,5-diene-1,4-dione

ChemComp-CRT:
SPIRILLOXANTHIN

ChemComp-CDL:
CARDIOLIPIN / phospholipid*YM

ChemComp-PEF:
DI-PALMITOYL-3-SN-PHOSPHATIDYLETHANOLAMINE / phospholipid*YM

Source
  • rhodospirillum rubrum (bacteria)
  • rhodospirillum rubrum (strain atcc 11170 / ath 1.1.1 / dsm 467 / lmg 4362 / ncib 8255 / s1) (bacteria)
KeywordsPHOTOSYNTHESIS / LH1-RC COMPLEX / PURPLE BACTERIA

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