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TitleStructural insights into viral RNA capping and plasma membrane targeting by Chikungunya virus nonstructural protein 1.
Journal, issue, pagesCell Host Microbe, Vol. 29, Issue 5, Page 757-764.e3, Year 2021
Publish dateMay 12, 2021
AuthorsKuo Zhang / Yee-Song Law / Michelle Cheok Yien Law / Yaw Bia Tan / Melissa Wirawan / Dahai Luo /
PubMed AbstractChikungunya virus (CHIKV) causes a debilitating arthralgic inflammatory disease in humans. The multifunctional CHIKV protein, nsP1, facilitates virus RNA replication and transcription by anchoring ...Chikungunya virus (CHIKV) causes a debilitating arthralgic inflammatory disease in humans. The multifunctional CHIKV protein, nsP1, facilitates virus RNA replication and transcription by anchoring the viral replication complex (RC) to plasma membrane vesicles and synthesizing the viral RNA 5' cap-0. Here, we report a cryo-EM structure of CHIKV nsP1 at 2.38 Å resolution. Twelve copies of nsP1 form a crown-shaped ring structure with a 7.5-nm-wide channel for mediating communication and exchange between the viral RC and the host cell. The catalytic site for viral RNA capping is located in a tunnel that is shaped by neighboring nsP1 molecules. Two membrane-association loops target nsP1 to the inner leaflet of the plasma membrane via palmitoylation and hydrophobic and electrostatic interactions. Our study provides the structural basis of viral RNA capping and RC assembly mediated by nsP1 and guides the development of antivirals targeting these essential steps of virus infection.
External linksCell Host Microbe / PubMed:33730549
MethodsEM (single particle)
Resolution2.38 Å
Structure data

30796

7dop

EMDB-30796, PDB-7dop:
Structural insights into viral RNA capping and plasma membrane targeting by Chikungunya virus nonstructural protein 1
Method: EM (single particle) / Resolution: 2.38 Å

Chemicals

ChemComp-ZN:
Unknown entry

Source
  • chikungunya virus strain s27-african prototype
KeywordsVIRAL PROTEIN / nonstructural protein / Chikungunya virus / RNA cap / replication / membrane associations

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