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Structure paper

TitleDirect pathogen-induced assembly of an NLR immune receptor complex to form a holoenzyme.
Journal, issue, pagesScience, Vol. 370, Issue 6521, Year 2020
Publish dateDec 4, 2020
AuthorsShoucai Ma / Dmitry Lapin / Li Liu / Yue Sun / Wen Song / Xiaoxiao Zhang / Elke Logemann / Dongli Yu / Jia Wang / Jan Jirschitzka / Zhifu Han / Paul Schulze-Lefert / Jane E Parker / Jijie Chai /
PubMed AbstractDirect or indirect recognition of pathogen-derived effectors by plant nucleotide-binding leucine-rich repeat (LRR) receptors (NLRs) initiates innate immune responses. The effector ATR1 activates the ...Direct or indirect recognition of pathogen-derived effectors by plant nucleotide-binding leucine-rich repeat (LRR) receptors (NLRs) initiates innate immune responses. The effector ATR1 activates the N-terminal Toll-interleukin-1 receptor (TIR) domain of NLR RPP1. We report a cryo-electron microscopy structure of RPP1 bound by ATR1. The structure reveals a C-terminal jelly roll/Ig-like domain (C-JID) for specific ATR1 recognition. Biochemical and functional analyses show that ATR1 binds to the C-JID and the LRRs to induce an RPP1 tetrameric assembly required for nicotinamide adenine dinucleotide hydrolase (NADase) activity. RPP1 tetramerization creates two potential active sites, each formed by an asymmetric TIR homodimer. Our data define the mechanism of direct effector recognition by a plant NLR leading to formation of a signaling-active holoenzyme.
External linksScience / PubMed:33273071
MethodsEM (single particle)
Resolution2.99 - 3.21 Å
Structure data

30449

7crb

EMDB-30449, PDB-7crb:
Cryo-EM structure of plant NLR RPP1 LRR-ID domain in complex with ATR1
Method: EM (single particle) / Resolution: 3.16 Å

30450

7crc

EMDB-30450, PDB-7crc:
Cryo-EM structure of plant NLR RPP1 tetramer in complex with ATR1
Method: EM (single particle) / Resolution: 3.02 Å

EMDB-30451:
Cryo-EM map of RPP1 mutant in complex with ATR1
Method: EM (single particle) / Resolution: 3.21 Å

30579

7dfv

EMDB-30579, PDB-7dfv:
Cryo-EM structure of plant NLR RPP1 tetramer core part
Method: EM (single particle) / Resolution: 2.99 Å

Chemicals

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

Source
  • hyaloperonospora arabidopsidis (strain emoy2) (eukaryote)
  • arabidopsis thaliana (thale cress)
  • Hyaloperonospora arabidopsidis Emoy2 (eukaryote)
KeywordsPLANT PROTEIN / NADase / ETI / HR

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