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TitleApple latent spherical virus structure with stable capsid frame supports quasi-stable protrusions expediting genome release.
Journal, issue, pagesCommun Biol, Vol. 3, Issue 1, Page 488, Year 2020
Publish dateSep 4, 2020
AuthorsHisashi Naitow / Tasuku Hamaguchi / Saori Maki-Yonekura / Masamichi Isogai / Nobuyuki Yoshikawa / Koji Yonekura /
PubMed AbstractPicorna-like plant viruses are non-enveloped RNA spherical viruses of ~30 nm. Part of the survival of these viruses depends on their capsid being stable enough to harbour the viral genome and yet ...Picorna-like plant viruses are non-enveloped RNA spherical viruses of ~30 nm. Part of the survival of these viruses depends on their capsid being stable enough to harbour the viral genome and yet malleable enough to allow its release. However, molecular mechanisms remain obscure. Here, we report a structure of a picorna-like plant virus, apple latent spherical virus, at 2.87 Å resolution by single-particle cryo-electron microscopy (cryo-EM) with a cold-field emission beam. The cryo-EM map reveals a unique structure composed of three capsid proteins Vp25, Vp20, and Vp24. Strikingly Vp25 has a long N-terminal extension, which substantially stabilises the capsid frame of Vp25 and Vp20 subunits. Cryo-EM images also resolve RNA genome leaking from a pentameric protrusion of Vp24 subunits. The structures and observations suggest that genome release occurs through occasional opening of the Vp24 subunits, possibly suppressed to a low frequency by the rigid frame of the other subunits.
External linksCommun Biol / PubMed:32887929 / PubMed Central
MethodsEM (single particle)
Resolution2.87 Å
Structure data

30375

7chk

EMDB-30375, PDB-7chk:
Cryo-EM Structure of Apple Latent Spherical Virus (ALSV)
Method: EM (single particle) / Resolution: 2.87 Å

Source
  • apple latent spherical virus
KeywordsSTRUCTURAL PROTEIN / Cheravirus / capsid stabilization / genome release / single particle cryo-EM

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