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TitleUnique active-site and subsite features in the arabinogalactan-degrading GH43 exo-beta-1,3-galactanase from Phanerochaete chrysosporium .
Journal, issue, pagesJ. Biol. Chem., Vol. 295, Page 18539-18552, Year 2020
Publish dateApr 24, 2020 (structure data deposition date)
AuthorsMatsuyama, K. / Kishine, N. / Fujimoto, Z. / Sunagawa, N. / Kotake, T. / Tsumuraya, Y. / Samejima, M. / Igarashi, K. / Kaneko, S.
External linksJ. Biol. Chem. / PubMed:33093171
MethodsX-ray diffraction
Resolution1.4 - 2.5 Å
Structure data

PDB-7bys:
Crystal structure of exo-beta-1,3-galactanase from Phanerochaete chrysosporium Pc1,3Gal43A apo form
Method: X-RAY DIFFRACTION / Resolution: 1.4 Å

PDB-7byt:
Crystal structure of exo-beta-1,3-galactanase from Phanerochaete chrysosporium Pc1,3Gal43A with galactose
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-7byv:
Crystal structure of exo-beta-1,3-galactanase from Phanerochaete chrysosporium Pc1,3Gal43A E208Q with beta-1,3-galactotriose
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-7byx:
Crystal structure of exo-beta-1,3-galactanase from Phanerochaete chrysosporium Pc1,3Gal43A E208A with beta-1,3-galactotriose
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

ChemComp-CA:
Unknown entry

ChemComp-CIT:
CITRIC ACID

ChemComp-HOH:
WATER

ChemComp-GLA:
alpha-D-galactopyranose

ChemComp-GAL:
beta-D-galactopyranose

ChemComp-ACY:
ACETIC ACID

ChemComp-ACT:
ACETATE ION

ChemComp-GOL:
GLYCEROL

Source
  • phanerochaete chrysosporium (fungus)
KeywordsHYDROLASE / Glycoside hydrolase family 43 / exo-beta-1 / 3-galactanase / arabinogalactan degrade / apo form / carbohydrate binding module family 35 / complex with galactotriose / complex with beta-1 / 3-galactotriose

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