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TitleCryo-EM structure of DyP-loaded encapsulin.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 118, Issue 16, Year 2021
Publish dateApr 20, 2021
AuthorsYanting Tang / An Mu / Yuying Zhang / Shan Zhou / Weiwei Wang / Yuezheng Lai / Xiaoting Zhou / Fengjiang Liu / Xiuna Yang / Hongri Gong / Quan Wang / Zihe Rao /
PubMed AbstractEncapsulins containing dye-decolorizing peroxidase (DyP)-type peroxidases are ubiquitous among prokaryotes, protecting cells against oxidative stress. However, little is known about how they interact ...Encapsulins containing dye-decolorizing peroxidase (DyP)-type peroxidases are ubiquitous among prokaryotes, protecting cells against oxidative stress. However, little is known about how they interact and function. Here, we have isolated a native cargo-packaging encapsulin from and determined its complete high-resolution structure by cryogenic electron microscopy (cryo-EM). This encapsulin comprises an icosahedral shell and a dodecameric DyP cargo. The dodecameric DyP consists of two hexamers with a twofold axis of symmetry and stretches across the interior of the encapsulin. Our results reveal that the encapsulin shell plays a role in stabilizing the dodecameric DyP. Furthermore, we have proposed a potential mechanism for removing the hydrogen peroxide based on the structural features. Our study also suggests that the DyP is the primary cargo protein of mycobacterial encapsulins and is a potential target for antituberculosis drug discovery.
External linksProc Natl Acad Sci U S A / PubMed:33853951 / PubMed Central
MethodsEM (single particle)
Resolution2.5 - 4.1 Å
Structure data

30130

7boj

EMDB-30130, PDB-7boj:
Cryo-EM structure of the encapsulin shell from Mycobacterium smegmatis
Method: EM (single particle) / Resolution: 2.5 Å

30131

7bok

EMDB-30131, PDB-7bok:
Cryo-EM structure of the encapsulated DyP-type peroxidase from Mycobacterium smegmatis
Method: EM (single particle) / Resolution: 3.7 Å

EMDB-30132:
Cryo-EM structure of the cargo-loaded encapsulin from Mycobacterium smegmatis
Method: EM (single particle) / Resolution: 4.1 Å

Chemicals

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE / Heme B

Source
  • mycolicibacterium smegmatis mc2 155 (bacteria)
KeywordsVIRUS LIKE PARTICLE / Nanocompartment / Icosahedral shell / Cargo loaded / OXIDOREDUCTASE / Cargo protein / Dodecamer / Heme-containing enzyme

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