Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	The coupling mechanism of mammalian respiratory complex I.
Journal, issue, pages	Science, Vol. 370, Issue 6516, Year 2020
Publish date	Oct 30, 2020
Authors	Domen Kampjut / Leonid A Sazanov /
PubMed Abstract	Mitochondrial complex I couples NADH:ubiquinone oxidoreduction to proton pumping by an unknown mechanism. Here, we present cryo-electron microscopy structures of ovine complex I in five different ...Mitochondrial complex I couples NADH:ubiquinone oxidoreduction to proton pumping by an unknown mechanism. Here, we present cryo-electron microscopy structures of ovine complex I in five different conditions, including turnover, at resolutions up to 2.3 to 2.5 angstroms. Resolved water molecules allowed us to experimentally define the proton translocation pathways. Quinone binds at three positions along the quinone cavity, as does the inhibitor rotenone that also binds within subunit ND4. Dramatic conformational changes around the quinone cavity couple the redox reaction to proton translocation during open-to-closed state transitions of the enzyme. In the induced deactive state, the open conformation is arrested by the ND6 subunit. We propose a detailed molecular coupling mechanism of complex I, which is an unexpected combination of conformational changes and electrostatic interactions.
External links	Science / PubMed:32972993
Methods	EM (single particle)
Resolution	2.3 - 3.8 Å
Structure data	11241 6zk9 EMDB-11241, PDB-6zk9: Peripheral domain of open complex I during turnover Method: EM (single particle) / Resolution: 2.3 Å 11242 6zka EMDB-11242, PDB-6zka: Membrane domain of open complex I during turnover Method: EM (single particle) / Resolution: 2.5 Å 11243 6zkb EMDB-11243, PDB-6zkb: Membrane domain of closed complex I during turnover Method: EM (single particle) / Resolution: 2.9 Å 11244 6zkc EMDB-11244, PDB-6zkc: Complex I during turnover, closed Method: EM (single particle) / Resolution: 3.1 Å 11245 6zkd EMDB-11245, PDB-6zkd: Complex I during turnover, open1 Method: EM (single particle) / Resolution: 2.7 Å 11246 6zke EMDB-11246, PDB-6zke: Complex I during turnover, open2 Method: EM (single particle) / Resolution: 2.6 Å 11247 6zkf EMDB-11247, PDB-6zkf: Complex I during turnover, open3 Method: EM (single particle) / Resolution: 2.8 Å 11248 6zkg EMDB-11248, PDB-6zkg: Complex I with NADH, closed Method: EM (single particle) / Resolution: 3.4 Å 11249 6zkh EMDB-11249, PDB-6zkh: Complex I with NADH, open1 Method: EM (single particle) / Resolution: 3.0 Å 11250 6zki EMDB-11250, PDB-6zki: Complex I with NADH, open2 Method: EM (single particle) / Resolution: 2.8 Å 11251 6zkj EMDB-11251, PDB-6zkj: Complex I with NADH, open3 Method: EM (single particle) / Resolution: 3.0 Å 11252 6zkk EMDB-11252, PDB-6zkk: Complex I inhibited by rotenone, closed Method: EM (single particle) / Resolution: 3.7 Å 11253 6zkl EMDB-11253, PDB-6zkl: Complex I inhibited by rotenone, open1 Method: EM (single particle) / Resolution: 3.1 Å 11254 6zkm EMDB-11254, PDB-6zkm: Complex I inhibited by rotenone, open2 Method: EM (single particle) / Resolution: 2.8 Å 11255 6zkn EMDB-11255, PDB-6zkn: Complex I inhibited by rotenone, open3 Method: EM (single particle) / Resolution: 2.9 Å 11256 6zko EMDB-11256, PDB-6zko: Native complex I, closed Method: EM (single particle) / Resolution: 3.8 Å 11257 6zkp EMDB-11257, PDB-6zkp: Native complex I, open1 Method: EM (single particle) / Resolution: 3.2 Å 11258 6zkq EMDB-11258, PDB-6zkq: Native complex I, open2 Method: EM (single particle) / Resolution: 3.3 Å 11259 6zkr EMDB-11259, PDB-6zkr: Native complex I, open3 Method: EM (single particle) / Resolution: 3.5 Å 11260 6zks EMDB-11260, PDB-6zks: Deactive complex I, open1 Method: EM (single particle) / Resolution: 3.1 Å 11261 6zkt EMDB-11261, PDB-6zkt: Deactive complex I, open2 Method: EM (single particle) / Resolution: 2.8 Å 11262 6zku EMDB-11262, PDB-6zku: Deactive complex I, open3 Method: EM (single particle) / Resolution: 3.0 Å 11263 6zkv EMDB-11263, PDB-6zkv: Deactive complex I, open4 Method: EM (single particle) / Resolution: 2.9 Å EMDB-15216: Complex I inhibited by rotenone open3 Method: EM (single particle) / Resolution: 2.9 Å
Chemicals	ChemComp-SF4: IRON/SULFUR CLUSTER ChemComp-FMN: FLAVIN MONONUCLEOTIDE ChemComp-NAI: 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE ChemComp-FES: FE2/S2 (INORGANIC) CLUSTER ChemComp-K: Unknown entry ChemComp-PC1: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / phospholipidYM ChemComp-3PE: 1,2-Distearoyl-sn-glycerophosphoethanolamine / phospholipidYM ChemComp-ZN: Unknown entry ChemComp-NDP: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE ChemComp-ZMP: S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate ChemComp-CDL: CARDIOLIPIN / phospholipidYM ChemComp-HOH: WATER ChemComp-DCQ: 2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione ChemComp-AMP: ADENOSINE MONOPHOSPHATE / AMPYM ChemComp-MYR: MYRISTIC ACID ChemComp-970: (2R,6aS,12aS)-8,9-dimethoxy-2-(prop-1-en-2-yl)-1,2,12,12a-tetrahydrofuro[2',3':7,8][1]benzopyrano[2,3-c][1]benzopyran-6(6aH)-one
Source	ovis aries (sheep) Sheep (sheep)
Keywords	ELECTRON TRANSPORT / complex / respiration / NADH / proton pump / mitochondria / iron-sulphur cluster / oxidoreductase / membrane protein