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Structure paper

TitleA comprehensive binding study illustrates ligand recognition in the periplasmic binding protein PotF.
Journal, issue, pagesStructure, Vol. 29, Page 433-, Year 2021
Publish dateMar 23, 2020 (structure data deposition date)
AuthorsKroger, P. / Shanmugaratnam, S. / Ferruz, N. / Schweimer, K. / Hocker, B.
External linksStructure / PubMed:33406388
MethodsX-ray diffraction
Resolution1.5 - 2.18 Å
Structure data

PDB-6ye0:
E.coli's Putrescine receptor PotF complexed with Putrescine
Method: X-RAY DIFFRACTION / Resolution: 1.63 Å

PDB-6ye6:
E.coli's Putrescine receptor PotF complexed with Agmatine
Method: X-RAY DIFFRACTION / Resolution: 1.56 Å

PDB-6ye7:
E.coli's Putrescine receptor PotF complexed with Cadaverine
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

PDB-6ye8:
E.coli's Putrescine receptor PotF complexed with Spermidine
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-6yeb:
E.coli's Putrescine receptor PotF in its closed apo state
Method: X-RAY DIFFRACTION / Resolution: 1.97 Å

PDB-6yec:
E.coli's Putrescine receptor PotF complexed with Spermine
Method: X-RAY DIFFRACTION / Resolution: 2.09 Å

PDB-6yed:
E.coli's Putrescine receptor PotF in its open apo state
Method: X-RAY DIFFRACTION / Resolution: 2.18 Å

Chemicals

ChemComp-PUT:
1,4-DIAMINOBUTANE

ChemComp-JFN:
(2R)-1-methoxypropan-2-amine

ChemComp-ONW:
(2~{R})-1-[(2~{R})-1-(2-methoxyethoxy)propan-2-yl]oxypropan-2-amine

ChemComp-ONT:
(2~{S})-1-(2-methoxyethoxy)propan-2-amine

ChemComp-HOH:
WATER

ChemComp-AG2:
AGMATINE

ChemComp-SO4:
SULFATE ION

ChemComp-CL:
Unknown entry

ChemComp-N2P:
PENTANE-1,5-DIAMINE

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-PG4:
TETRAETHYLENE GLYCOL / precipitant*YM

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-PGE:
TRIETHYLENE GLYCOL

ChemComp-TAM:
TRIS(HYDROXYETHYL)AMINOMETHANE / pH buffer*YM

ChemComp-P33:
3,6,9,12,15,18-HEXAOXAICOSANE-1,20-DIOL / precipitant*YM

ChemComp-MLI:
MALONATE ION

ChemComp-1PE:
PENTAETHYLENE GLYCOL / precipitant*YM

ChemComp-SPD:
SPERMIDINE

ChemComp-GOL:
GLYCEROL

ChemComp-SPM:
SPERMINE

Source
  • escherichia coli (strain k12) (bacteria)
  • escherichia coli k-12 (bacteria)
  • escherichia coli k12 (bacteria)
KeywordsTRANSPORT PROTEIN / Periplasmic binding protein / E.coli / Putrescine / Agmatine / Cadaverine / Spermidine / closed apo / Spermine / open apo

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