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TitleHigh resolution CryoEM structure of the ring-shaped virulence factor EspB from .
Journal, issue, pagesJ Struct Biol X, Vol. 4, Page 100029, Year 2020
Publish dateJul 2, 2020
AuthorsJérémie Piton / Florence Pojer / Soichi Wakatsuki / Cornelius Gati / Stewart T Cole /
PubMed AbstractThe EspB protein of is a 60 kDa virulence factor, implicated in conjugation and exported by the ESX-1 system of which it may also be a component. Previous attempts to obtain high-resolution maps of ...The EspB protein of is a 60 kDa virulence factor, implicated in conjugation and exported by the ESX-1 system of which it may also be a component. Previous attempts to obtain high-resolution maps of EspB by cryo-electron microscopic examination of single particles have been thwarted by severe orientation bias of the particles. This was overcome by using detergent as a surfactant thereby allowing reconstruction of the EspB structure at 3.37 Å resolution. The final structure revealed the N-terminal domain of EspB to be organized as a cylindrical heptamer with dimensions of 90 Å x 90 Å and a central channel of 45 Å diameter whereas the C-terminal domain was unstructured. New atomic insight was obtained into the helical packing required for protomer interactions and the overall electrostatic potential. The external surface is electronegatively charged while the channel is lined with electropositive patches. EspB thus has many features of a pore-like transport protein that might allow the passage of an ESX-1 substrate such as the 35 Å diameter EsxA-EsxB heterodimer or B-form DNA consistent with its proposed role in DNA uptake.
External linksJ Struct Biol X / PubMed:32875288 / PubMed Central
MethodsEM (single particle)
Resolution3.37 Å
Structure data

10658

6xzc

EMDB-10658, PDB-6xzc:
CryoEM structure of the ring-shaped virulence factor EspB from Mycobacterium tuberculosis
Method: EM (single particle) / Resolution: 3.37 Å

Source
  • Mycobacterium tuberculosis H37Rv (bacteria)
  • mycobacterium tuberculosis (strain atcc 25618 / h37rv) (bacteria)
KeywordsTRANSPORT PROTEIN / M. tuberculosis / ESX-1 / Type VII secretion system / EspB / Rv3881c / EsxA / CFP10 / ESAT-6

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