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TitleStructural and Functional Analysis of the D614G SARS-CoV-2 Spike Protein Variant.
Journal, issue, pagesCell, Vol. 183, Issue 3, Page 739-751.e8, Year 2020
Publish dateOct 29, 2020
AuthorsLeonid Yurkovetskiy / Xue Wang / Kristen E Pascal / Christopher Tomkins-Tinch / Thomas P Nyalile / Yetao Wang / Alina Baum / William E Diehl / Ann Dauphin / Claudia Carbone / Kristen Veinotte / Shawn B Egri / Stephen F Schaffner / Jacob E Lemieux / James B Munro / Ashique Rafique / Abhi Barve / Pardis C Sabeti / Christos A Kyratsous / Natalya V Dudkina / Kuang Shen / Jeremy Luban /
PubMed AbstractThe SARS-CoV-2 spike (S) protein variant D614G supplanted the ancestral virus worldwide, reaching near fixation in a matter of months. Here we show that D614G was more infectious than the ancestral ...The SARS-CoV-2 spike (S) protein variant D614G supplanted the ancestral virus worldwide, reaching near fixation in a matter of months. Here we show that D614G was more infectious than the ancestral form on human lung cells, colon cells, and on cells rendered permissive by ectopic expression of human ACE2 or of ACE2 orthologs from various mammals, including Chinese rufous horseshoe bat and Malayan pangolin. D614G did not alter S protein synthesis, processing, or incorporation into SARS-CoV-2 particles, but D614G affinity for ACE2 was reduced due to a faster dissociation rate. Assessment of the S protein trimer by cryo-electron microscopy showed that D614G disrupts an interprotomer contact and that the conformation is shifted toward an ACE2 binding-competent state, which is modeled to be on pathway for virion membrane fusion with target cells. Consistent with this more open conformation, neutralization potency of antibodies targeting the S protein receptor-binding domain was not attenuated.
External linksCell / PubMed:32991842 / PubMed Central
MethodsEM (single particle)
Resolution3.7 Å
Structure data

22301

6xs6

EMDB-22301, PDB-6xs6:
SARS-CoV-2 Spike D614G variant, minus RBD
Method: EM (single particle) / Resolution: 3.7 Å

Source
  • severe acute respiratory syndrome coronavirus 2
KeywordsVIRAL PROTEIN / SARS-CoV-2 / Spike / D614G

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