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TitleDXO/Rai1 enzymes remove 5'-end FAD and dephospho-CoA caps on RNAs.
Journal, issue, pagesNucleic Acids Res., Vol. 48, Page 6136-6148, Year 2020
Publish dateMay 4, 2020 (structure data deposition date)
AuthorsDoamekpor, S.K. / Grudzien-Nogalska, E. / Mlynarska-Cieslak, A. / Kowalska, J. / Kiledjian, M. / Tong, L.
External linksNucleic Acids Res. / PubMed:32374864
MethodsX-ray diffraction
Resolution1.599 - 1.9 Å
Structure data

PDB-6wuf:
Crystal structure of mouse DXO in complex with 3'-FADP
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

PDB-6wug:
Crystal Structure of S. pombe Rai1 in complex with 3'-FADP
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-6wui:
Crystal Structure of mutant S. pombe Rai1 (E150S/E199Q/E239Q) in complex with 3'-FADP
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-6wuk:
Crystal structure of mouse DXO in complex with CoA
Method: X-RAY DIFFRACTION / Resolution: 1.599 Å

Chemicals

ChemComp-UBG:
[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-4-hydroxy-3-(phosphonooxy)tetrahydrofuran-2-yl]methyl (2R,3S,4S)-5-(7,8-dimethyl-2,4-dioxo-3,4-dihydrobenzo[g]pteridin-10(2H)-yl)-2,3,4-trihydroxypentyl dihydrogen diphosphate (non-preferred name)

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-HOH:
WATER

ChemComp-MG:
Unknown entry

ChemComp-COA:
COENZYME A

Source
  • mus musculus (house mouse)
  • schizosaccharomyces pombe (fission yeast)
KeywordsHYDROLASE / FAD / 3'-FADP / RNA / cap / decapping / Rai1 / DXO / CoA / Coenzyme A

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