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Structure paper

TitleOvercoming Near-Cognate Suppression in a Release Factor 1-Deficient Host with an Improved Nitro-Tyrosine tRNA Synthetase.
Journal, issue, pagesJ. Mol. Biol., Vol. 432, Page 4690-4704, Year 2020
Publish dateApr 29, 2020 (structure data deposition date)
AuthorsBeyer, J.N. / Hosseinzadeh, P. / Gottfried-Lee, I. / Van Fossen, E.M. / Zhu, P. / Bednar, R.M. / Karplus, P.A. / Mehl, R.A. / Cooley, R.B.
External linksJ. Mol. Biol. / PubMed:32569745
MethodsX-ray diffraction
Resolution1.55 - 1.95 Å
Structure data

PDB-6wrk:
Crystal structure of 3rd-generation Mj 3-nitro-tyrosine tRNA synthetase ("A7") bound to 3-nitro-tyrosine
Method: X-RAY DIFFRACTION / Resolution: 1.95 Å

PDB-6wrn:
Crystal structure of Mj 3-nitro-tyrosine tRNA synthetase (5B) C70A variant bound to 3-nitro-tyrosine
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

PDB-6wrq:
Crystal structure of Mj 3-nitro-tyrosine tRNA synthetase (5B) S158C variant bound to 3-nitro-tyrosine
Method: X-RAY DIFFRACTION / Resolution: 1.85 Å

PDB-6wrt:
Crystal structure of Mj 3-nitro-tyrosine tRNA synthetase (5B) C70A/S158C variant bound to 3-nitro-tyrosine
Method: X-RAY DIFFRACTION / Resolution: 1.55 Å

Chemicals

ChemComp-GOL:
GLYCEROL

ChemComp-NA:
Unknown entry

ChemComp-NIY:
META-NITRO-TYROSINE

ChemComp-HOH:
WATER

Source
  • methanocaldococcus jannaschii (strain atcc 43067 / dsm 2661 / jal-1 / jcm 10045 / nbrc 100440) (archaea)
KeywordsLIGASE / aminoacyl-tRNA synthetase / 3-nitro-tyrosine

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