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TitleA glycoprotein B-neutralizing antibody structure at 2.8 Å uncovers a critical domain for herpesvirus fusion initiation.
Journal, issue, pagesNat Commun, Vol. 11, Issue 1, Page 4141, Year 2020
Publish dateAug 18, 2020
AuthorsStefan L Oliver / Yi Xing / Dong-Hua Chen / Soung Hun Roh / Grigore D Pintilie / David A Bushnell / Marvin H Sommer / Edward Yang / Andrea Carfi / Wah Chiu / Ann M Arvin /
PubMed AbstractMembers of the Herpesviridae, including the medically important alphaherpesvirus varicella-zoster virus (VZV), induce fusion of the virion envelope with cell membranes during entry, and between cells ...Members of the Herpesviridae, including the medically important alphaherpesvirus varicella-zoster virus (VZV), induce fusion of the virion envelope with cell membranes during entry, and between cells to form polykaryocytes in infected tissues. The conserved glycoproteins, gB, gH and gL, are the core functional proteins of the herpesvirus fusion complex. gB serves as the primary fusogen via its fusion loops, but functions for the remaining gB domains remain unexplained. As a pathway for biological discovery of domain function, our approach used structure-based analysis of the viral fusogen together with a neutralizing antibody. We report here a 2.8 Å cryogenic-electron microscopy structure of native gB recovered from VZV-infected cells, in complex with a human monoclonal antibody, 93k. This high-resolution structure guided targeted mutagenesis at the gB-93k interface, providing compelling evidence that a domain spatially distant from the gB fusion loops is critical for herpesvirus fusion, revealing a potential new target for antiviral therapies.
External linksNat Commun / PubMed:32811830 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution2.4529 - 2.8 Å
Structure data

21247

6vn1

EMDB-21247, PDB-6vn1:
A 2.8 Angstrom Cryo-EM Structure of a Glycoprotein B-Neutralizing Antibody Complex Reveals a Critical Domain for Herpesvirus Fusion Initiation
Method: EM (single particle) / Resolution: 2.8 Å

PDB-6vlk:
A varicella-zoster virus glycoprotein
Method: X-RAY DIFFRACTION / Resolution: 2.4529 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

ChemComp-HOH:
WATER

Source
  • homo sapiens (human)
  • Human herpesvirus 3 (Varicella-zoster virus)
  • HHV-3, Varicella zoster virus, Human alphaherpesvirus 3
  • varicella-zoster virus (strain oka vaccine)
  • human alphaherpesvirus 3 (Varicella-zoster virus)
KeywordsVIRAL PROTEIN / viral membrane glycoprotein / VIRAL PROTEIN/IMMUNE SYSTEM / Varicella Zoster Virus / Glycoprotein B / Neutralization / Monoclonal Antibody / VIRAL PROTEIN-IMMUNE SYSTEM complex

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