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TitleDNA clamp function of the monoubiquitinated Fanconi anaemia ID complex.
Journal, issue, pagesNature, Vol. 580, Issue 7802, Page 278-282, Year 2020
Publish dateMar 11, 2020
AuthorsRenjing Wang / Shengliu Wang / Ankita Dhar / Christopher Peralta / Nikola P Pavletich /
PubMed AbstractThe ID complex, involving the proteins FANCI and FANCD2, is required for the repair of DNA interstrand crosslinks (ICL) and related lesions. These proteins are mutated in Fanconi anaemia, a disease ...The ID complex, involving the proteins FANCI and FANCD2, is required for the repair of DNA interstrand crosslinks (ICL) and related lesions. These proteins are mutated in Fanconi anaemia, a disease in which patients are predisposed to cancer. The Fanconi anaemia pathway of ICL repair is activated when a replication fork stalls at an ICL; this triggers monoubiquitination of the ID complex, in which one ubiquitin molecule is conjugated to each of FANCI and FANCD2. Monoubiquitination of ID is essential for ICL repair by excision, translesion synthesis and homologous recombination; however, its function remains unknown. Here we report a cryo-electron microscopy structure of the monoubiquitinated human ID complex bound to DNA, and reveal that it forms a closed ring that encircles the DNA. By comparison with the structure of the non-ubiquitinated ID complex bound to ICL DNA-which we also report here-we show that monoubiquitination triggers a complete rearrangement of the open, trough-like ID structure through the ubiquitin of one protomer binding to the other protomer in a reciprocal fashion. These structures-together with biochemical data-indicate that the monoubiquitinated ID complex loses its preference for ICL and related branched DNA structures, and becomes a sliding DNA clamp that can coordinate the subsequent repair reactions. Our findings also reveal how monoubiquitination in general can induce an alternative protein structure with a new function.
External linksNature / PubMed:32269332 / PubMed Central
MethodsEM (single particle)
Resolution3.3 - 3.9 Å
Structure data

21134

6vaa

EMDB-21134, PDB-6vaa:
Structure of the Fanconi Anemia ID complex bound to ICL DNA
Method: EM (single particle) / Resolution: 3.4 Å

21137

6vad

EMDB-21137, PDB-6vad:
Fanconi Anemia ID complex
Method: EM (single particle) / Resolution: 3.3 Å

21138

6vae

EMDB-21138, PDB-6vae:
Mono-ubiquitinated Fanconi Anemia ID complex bound to ICL DNA
Method: EM (single particle) / Resolution: 3.6 Å

21139

6vaf

EMDB-21139: Structure of mono-ubiquitinated FANCD2 bound to non-ubiquitinated FANCI and to DNA.
PDB-6vaf: Structure of mono-ubiquitinated FANCD2 bound to non-ubiquitinated FANCI and to DNA
Method: EM (single particle) / Resolution: 3.9 Å

Source
  • homo sapiens (human)
KeywordsDNA BINDING PROTEIN/DNA / DNA repair / DNA BINDING PROTEIN / DNA BINDING PROTEIN-DNA complex / DNA clamp

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