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Structure paper

TitleRNA extension drives a stepwise displacement of an initiation-factor structural module in initial transcription.
Journal, issue, pagesProc. Natl. Acad. Sci. USA, Vol. 117, Page 5801-5809, Year 2020
Publish dateAug 12, 2019 (structure data deposition date)
AuthorsLi, L. / Molodtsov, V. / Lin, W. / Ebright, R.H. / Zhang, Y.
External linksProc. Natl. Acad. Sci. USA / PubMed:32127479
MethodsX-ray diffraction
Resolution2.45 - 3.8 Å
Structure data

PDB-6kon:
Mycobacterium tuberculosis initial transcription complex comprising sigma H and 5'-OH RNA of 5 nt
Method: X-RAY DIFFRACTION / Resolution: 3 Å

PDB-6koo:
Mycobacterium tuberculosis initial transcription complex comprising sigma H and 5'-OH RNA of 7 nt
Method: X-RAY DIFFRACTION / Resolution: 2.8 Å

PDB-6kop:
Mycobacterium tuberculosis initial transcription complex comprising sigma H and 5'-OH RNA of 9 nt
Method: X-RAY DIFFRACTION / Resolution: 3.303 Å

PDB-6koq:
Mycobacterium tuberculosis initial transcription complex comprising sigma H and 5'-OH RNA of 10 nt
Method: X-RAY DIFFRACTION / Resolution: 3.353 Å

PDB-6kqd:
Thermus thermophilus initial transcription complex comprising sigma A and 5'-OH RNA of 3 nt
Method: X-RAY DIFFRACTION / Resolution: 3.3 Å

PDB-6kqe:
Thermus thermophilus initial transcription complex comprising sigma A and 5'-OH RNA of 4 nt
Method: X-RAY DIFFRACTION / Resolution: 3.3 Å

PDB-6kqf:
Thermus thermophilus initial transcription complex comprising sigma A and 5'-OH RNA of 5 nt
Method: X-RAY DIFFRACTION / Resolution: 2.45 Å

PDB-6kqg:
Thermus thermophilus initial transcription complex comprising sigma A and 5'-OH RNA of 6 nt
Method: X-RAY DIFFRACTION / Resolution: 2.783 Å

PDB-6kqh:
Thermus thermophilus initial transcription complex comprising sigma A and 5'-OH RNA of 7 nt
Method: X-RAY DIFFRACTION / Resolution: 3.18 Å

PDB-6kql:
Thermus thermophilus initial transcription complex comprising sigma A and 5'-triphosphate RNA of 4 nt
Method: X-RAY DIFFRACTION / Resolution: 2.89 Å

PDB-6kqm:
Thermus thermophilus initial transcription complex comprising sigma A and 5'-triphosphate RNA of 5 nt
Method: X-RAY DIFFRACTION / Resolution: 3.197 Å

PDB-6kqn:
Thermus thermophilus initial transcription complex comprising sigma A and 5'-triphosphate RNA of 6 nt
Method: X-RAY DIFFRACTION / Resolution: 3.489 Å

PDB-6l74:
Thermus thermophilus initial transcription complex comprising sigma A and 5'-triphosphate RNA of 2 nt
Method: X-RAY DIFFRACTION / Resolution: 3.12 Å

PDB-6lts:
Crystal structure of Thermus thermophilus transcription initiation complex comprising a truncated sigma finger
Method: X-RAY DIFFRACTION / Resolution: 3.45 Å

PDB-6tye:
Crystal structure of MTB sigma L transcription initiation complex with 5 nt long RNA primer
Method: X-RAY DIFFRACTION / Resolution: 3.79 Å

PDB-6tyf:
Crystal structure of MTB sigma L transcription initiation complex with 6 nt long RNA primer
Method: X-RAY DIFFRACTION / Resolution: 3.8 Å

PDB-6tyg:
Crystal structure of MTB sigma L transcription initiation complex with 9 nt long RNA primer
Method: X-RAY DIFFRACTION / Resolution: 3.5 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-DGP:
2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE

Source
  • mycobacterium tuberculosis h37rv (bacteria)
  • synthetic construct (others)
  • mycobacterium tuberculosis (strain atcc 25618 / h37rv) (bacteria)
  • thermus thermophilus (strain hb8 / atcc 27634 / dsm 579) (bacteria)
  • thermus thermophilus hb8 (bacteria)
  • thermus thermophilus (bacteria)
  • mycobacterium tuberculosis (bacteria)
KeywordsTRANSCRIPTION / Mycobacterium tuberculosis / RNA polymerase / ECF sigma factor sigH / 7nt RNA / ECF sigma factor / 9nt RNA / Thermus thermophilus / initiation transcription complex / 3nt RNA / TRANSLATION / transcription initiation / 4nt RNA / transcription initiation complex / 5nt RNA / 6nt RNA / 5'-triphosphate / 5'-triphosphate 5nt RNA / 5'-triphosphate 6nt RNA / 2nt RNA / sigma factor / bacterial transcription / initiation factor / sigma finger / tuberculosis / initiation / sigma finger displacement

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