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Structure paper

TitleA baculoviral system for the production of human beta-glucocerebrosidase enables atomic resolution analysis.
Journal, issue, pagesActa Crystallogr D Struct Biol, Vol. 76, Page 565-580, Year 2020
Publish dateNov 26, 2019 (structure data deposition date)
AuthorsRowland, R.J. / Wu, L. / Liu, F. / Davies, G.J.
External linksActa Crystallogr D Struct Biol / PubMed:32496218
MethodsX-ray diffraction
Resolution0.98 - 1.59 Å
Structure data

PDB-6tjj:
Structure of Cerezyme at pH 4.6
Method: X-RAY DIFFRACTION / Resolution: 1.59 Å

PDB-6tjk:
Crystal Structure of Recombinant GBA in Complex with Bis-Tris Propane.
Method: X-RAY DIFFRACTION / Resolution: 1.56 Å

PDB-6tjq:
Crystal Structure of Recombinant GBA in Complex with 2-Deoxy-2-fluoro-beta-D-glucopyranoside
Method: X-RAY DIFFRACTION / Resolution: 1.41 Å

PDB-6tn1:
Unliganded Crystal Structure of Recombinant GBA
Method: X-RAY DIFFRACTION / Resolution: 0.98 Å

Chemicals

ChemComp-SO4:
SULFATE ION

ChemComp-ACT:
ACETATE ION

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

ChemComp-K:
Unknown entry

ChemComp-NA:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-B3P:
2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH buffer*YM

ChemComp-NF8:
(2~{R},3~{S},4~{S},5~{S})-5-fluoranyl-2-(hydroxymethyl)oxane-3,4-diol

ChemComp-GLN:
GLUTAMINE

ChemComp-FMT:
FORMIC ACID

ChemComp-MG:
Unknown entry

Source
  • homo sapiens (human)
KeywordsHYDROLASE / beta-glucocerebrosidase / lysosomal glycoside hydrolase / GH30

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