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TitleStructure and dynamics of the E. coli chemotaxis core signaling complex by cryo-electron tomography and molecular simulations.
Journal, issue, pagesCommun Biol, Vol. 3, Issue 1, Page 24, Year 2020
Publish dateJan 10, 2020
AuthorsC Keith Cassidy / Benjamin A Himes / Dapeng Sun / Jun Ma / Gongpu Zhao / John S Parkinson / Phillip J Stansfeld / Zaida Luthey-Schulten / Peijun Zhang /
PubMed AbstractTo enable the processing of chemical gradients, chemotactic bacteria possess large arrays of transmembrane chemoreceptors, the histidine kinase CheA, and the adaptor protein CheW, organized as ...To enable the processing of chemical gradients, chemotactic bacteria possess large arrays of transmembrane chemoreceptors, the histidine kinase CheA, and the adaptor protein CheW, organized as coupled core-signaling units (CSU). Despite decades of study, important questions surrounding the molecular mechanisms of sensory signal transduction remain unresolved, owing especially to the lack of a high-resolution CSU structure. Here, we use cryo-electron tomography and sub-tomogram averaging to determine a structure of the Escherichia coli CSU at sub-nanometer resolution. Based on our experimental data, we use molecular simulations to construct an atomistic model of the CSU, enabling a detailed characterization of CheA conformational dynamics in its native structural context. We identify multiple, distinct conformations of the critical P4 domain as well as asymmetries in the localization of the P3 bundle, offering several novel insights into the CheA signaling mechanism.
External linksCommun Biol / PubMed:31925330 / PubMed Central
MethodsEM (subtomogram averaging)
Resolution8.38 - 8.4 Å
Structure data

10050

6s1k

EMDB-10050: Structure of the E. coli Chemotaxis Core Signaling Unit
PDB-6s1k: E. coli Core Signaling Unit, carrying QQQQ receptor mutation
Method: EM (subtomogram averaging) / Resolution: 8.4 Å

Source
  • Escherichia coli (E. coli)
  • escherichia coli str. k-12 substr. mg1655star (bacteria)
KeywordsSIGNALING PROTEIN / chemotaxis / methyl-accepting chemotaxis protein / histidine kinase

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