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TitleSolving a new R2lox protein structure by microcrystal electron diffraction.
Journal, issue, pagesSci Adv, Vol. 5, Issue 8, Page eaax4621, Year 2019
Publish dateAug 7, 2019
AuthorsHongyi Xu / Hugo Lebrette / Max T B Clabbers / Jingjing Zhao / Julia J Griese / Xiaodong Zou / Martin Högbom /
PubMed AbstractMicrocrystal electron diffraction (MicroED) has recently shown potential for structural biology. It enables the study of biomolecules from micrometer-sized 3D crystals that are too small to be ...Microcrystal electron diffraction (MicroED) has recently shown potential for structural biology. It enables the study of biomolecules from micrometer-sized 3D crystals that are too small to be studied by conventional x-ray crystallography. However, to date, MicroED has only been applied to redetermine protein structures that had already been solved previously by x-ray diffraction. Here, we present the first new protein structure-an R2lox enzyme-solved using MicroED. The structure was phased by molecular replacement using a search model of 35% sequence identity. The resulting electrostatic scattering potential map at 3.0-Å resolution was of sufficient quality to allow accurate model building and refinement. The dinuclear metal cofactor could be located in the map and was modeled as a heterodinuclear Mn/Fe center based on previous studies. Our results demonstrate that MicroED has the potential to become a widely applicable tool for revealing novel insights into protein structure and function.
External linksSci Adv / PubMed:31457106 / PubMed Central
MethodsEM (electron crystallography)
Resolution3 Å
Structure data

PDB-6qrz:
Crystal structure of R2-like ligand-binding oxidase from Sulfolobus acidocaldarius solved by 3D micro-crystal electron diffraction
Method: ELECTRON CRYSTALLOGRAPHY / Resolution: 3.0 Å

Chemicals

ChemComp-MN3:
MANGANESE (III) ION

ChemComp-FE:
Unknown entry

Source
  • sulfolobus acidocaldarius dsm 639 (acidophilic)
KeywordsOXIDOREDUCTASE / micro-crystal / microED / R2-LIKE LIGAND-BINDING OXIDASE / MN/FE COFACTOR / RIBONUCLEOTIDE REDUCTASE R2 SUBUNIT FOLD / METALLOPROTEIN OXIDOREDUCTASE / FERRITIN-LIKE SUPERFAMILY

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