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TitleStructure of the eukaryotic protein O-mannosyltransferase Pmt1-Pmt2 complex.
Journal, issue, pagesNat Struct Mol Biol, Vol. 26, Issue 8, Page 704-711, Year 2019
Publish dateJul 8, 2019
AuthorsLin Bai / Amanda Kovach / Qinglong You / Alanna Kenny / Huilin Li /
PubMed AbstractIn eukaryotes, a nascent peptide entering the endoplasmic reticulum (ER) is scanned by two Sec61 translocon-associated large membrane machines for protein N-glycosylation and protein O-mannosylation, ...In eukaryotes, a nascent peptide entering the endoplasmic reticulum (ER) is scanned by two Sec61 translocon-associated large membrane machines for protein N-glycosylation and protein O-mannosylation, respectively. While the structure of the eight-protein oligosaccharyltransferase complex has been determined recently, the structures of mannosyltransferases of the PMT family, which are an integral part of ER protein homeostasis, are still unknown. Here we report cryo-EM structures of the Saccharomyces cerevisiae Pmt1-Pmt2 complex bound to a donor and an acceptor peptide at 3.2-Å resolution, showing that each subunit contains 11 transmembrane helices and a lumenal β-trefoil fold termed the MIR domain. The structures reveal the substrate recognition model and confirm an inverting mannosyl-transferring reaction mechanism by the enzyme complex. Furthermore, we found that the transmembrane domains of Pmt1 and Pmt2 share a structural fold with the catalytic subunits of oligosaccharyltransferases, confirming a previously proposed evolutionary relationship between protein O-mannosylation and protein N-glycosylation.
External linksNat Struct Mol Biol / PubMed:31285605 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution1.35 - 3.2 Å
Structure data

20236

6p25

EMDB-20236, PDB-6p25:
Structure of S. cerevisiae protein O-mannosyltransferase Pmt1-Pmt2 complex bound to the sugar donor and a peptide acceptor
Method: EM (single particle) / Resolution: 3.2 Å

20240

6p2r

EMDB-20240, PDB-6p2r:
Structure of S. cerevisiae protein O-mannosyltransferase Pmt1-Pmt2 complex bound to the sugar donor
Method: EM (single particle) / Resolution: 3.2 Å

PDB-6p28:
Crystal structure of the MIR domain (aa 337-532) of the S. cerevisiae mannosyltransferase Pmt2
Method: X-RAY DIFFRACTION / Resolution: 1.35 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose / N-Acetylglucosamine

ChemComp-CPL:
1-PALMITOYL-2-LINOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE / phospholipid*YM / Phosphatidylcholine

ChemComp-NNM:
(3R)-3,31-dimethyl-7,11,15,19,23,27-hexamethylidenedotriacont-31-en-1-yl dihydrogen phosphate

ChemComp-HOH:
WATER / Water

Source
  • saccharomyces cerevisiae w303 (yeast)
  • saccharomyces cerevisiae (brewer's yeast)
KeywordsTRANSFERASE / complex / glycosylation

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