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Structure paper

TitleStructural basis of Q-dependent antitermination.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 116, Issue 37, Page 18384-18390, Year 2019
Publish dateSep 10, 2019
AuthorsZhou Yin / Jason T Kaelber / Richard H Ebright /
PubMed AbstractLambdoid bacteriophage Q protein mediates the switch from middle to late bacteriophage gene expression by enabling RNA polymerase (RNAP) to read through transcription terminators preceding ...Lambdoid bacteriophage Q protein mediates the switch from middle to late bacteriophage gene expression by enabling RNA polymerase (RNAP) to read through transcription terminators preceding bacteriophage late genes. Q loads onto RNAP engaged in promoter-proximal pausing at a Q binding element (QBE) and adjacent sigma-dependent pause element (SDPE) to yield a Q-loading complex, and Q subsequently translocates with RNAP as a pausing-deficient, termination-deficient Q-loaded complex. Here, we report high-resolution structures of 4 states on the pathway of antitermination by Q from bacteriophage 21 (Q21): Q21, the Q21-QBE complex, the Q21-loading complex, and the Q21-loaded complex. The results show that Q21 forms a torus, a "nozzle," that narrows and extends the RNAP RNA-exit channel, extruding topologically linked single-stranded RNA and preventing the formation of pause and terminator hairpins.
External linksProc Natl Acad Sci U S A / PubMed:31455742 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution1.942 - 3.8 Å
Structure data

20233

6p18

EMDB-20233, PDB-6p18:
Q21 transcription antitermination complex: loading complex
Method: EM (single particle) / Resolution: 3.5 Å

20234

6p19

EMDB-20234, PDB-6p19:
Q21 transcription antitermination complex: loaded complex
Method: EM (single particle) / Resolution: 3.8 Å

PDB-6p1a:
Transcription antitermination factor Q21 in complex with Q21-binding-element DNA
Method: X-RAY DIFFRACTION / Resolution: 2.837 Å

PDB-6p1b:
Transcription antitermination factor Q21
Method: X-RAY DIFFRACTION / Resolution: 1.942 Å

PDB-6p1c:
Transcription antitermination factor Q21, SeMet-derivative
Method: X-RAY DIFFRACTION / Resolution: 2.005 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-MG:
Unknown entry

ChemComp-EDO:
1,2-ETHANEDIOL / Ethylene glycol

ChemComp-CL:
Unknown entry / Chloride

ChemComp-HOH:
WATER / Water

Source
  • escherichia coli (E. coli)
  • phage 21 (virus)
  • escherichia coli (strain k12) (bacteria)
KeywordsGENE REGULATION / RNA polymerase / DNA Binding / transcription / Q-dependent antitermination / Q antiterminator factor / Q antitermination factor

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