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TitleAn Unexpected Species Determined by X-ray Crystallography that May Represent an Intermediate in the Reaction Catalyzed by Quinolinate Synthase.
Journal, issue, pagesJ. Am. Chem. Soc., Vol. 141, Page 14142-14151, Year 2019
Publish dateJan 25, 2019 (structure data deposition date)
AuthorsEsakova, O.A. / Silakov, A. / Grove, T.L. / Warui, D.M. / Yennawar, N.H. / Booker, S.J.
External linksJ. Am. Chem. Soc. / PubMed:31390192
MethodsX-ray diffraction
Resolution1.6 - 2.2 Å
Structure data

PDB-6nso:
An Unexpected Intermediate in the Reaction Catalyzed by Quinolinate Synthase
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

PDB-6nsu:
Crystallographic Capture of Quinolinate Synthase (NadA) from Pyrococcus horikoshii in its Substrates and Product-Bound States
Method: X-RAY DIFFRACTION / Resolution: 2.15 Å

PDB-6or8:
An Unexpected Intermediate in the Reaction Catalyzed by Quinolinate Synthase
Method: X-RAY DIFFRACTION / Resolution: 1.65 Å

PDB-6ora:
An Unexpected Intermediate in the Reaction Catalyzed by Quinolinate Synthase
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

Chemicals

ChemComp-13P:
1,3-DIHYDROXYACETONEPHOSPHATE

ChemComp-SF4:
IRON/SULFUR CLUSTER

ChemComp-HOH:
WATER

ChemComp-DYA:
DIDEHYDROASPARTATE

ChemComp-5XR:
2-hydroxy-N-[(1S)-1-hydroxy-3-oxopropyl]-L-aspartic acid

ChemComp-PO4:
PHOSPHATE ION

ChemComp-ACT:
ACETATE ION

ChemComp-5XW:
(2~{Z})-2-(1-oxidanyl-3-oxidanylidene-propyl)iminobutanedioic acid

ChemComp-CL:
Unknown entry

Source
  • pyrococcus horikoshii (strain atcc 700860 / dsm 12428 / jcm 9974 / nbrc 100139 / ot-3) (archaea)
KeywordsTRANSFERASE / quinolinic acid / biosynthesis / biosynthesis of nicotinamide adenine dinucleotide

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