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TitleCryo-EM structure of the homohexameric T3SS ATPase-central stalk complex reveals rotary ATPase-like asymmetry.
Journal, issue, pagesNat Commun, Vol. 10, Issue 1, Page 626, Year 2019
Publish dateFeb 7, 2019
AuthorsDorothy D Majewski / Liam J Worrall / Chuan Hong / Claire E Atkinson / Marija Vuckovic / Nobuhiko Watanabe / Zhiheng Yu / Natalie C J Strynadka /
PubMed AbstractMany Gram-negative bacteria, including causative agents of dysentery, plague, and typhoid fever, rely on a type III secretion system - a multi-membrane spanning syringe-like apparatus - for their ...Many Gram-negative bacteria, including causative agents of dysentery, plague, and typhoid fever, rely on a type III secretion system - a multi-membrane spanning syringe-like apparatus - for their pathogenicity. The cytosolic ATPase complex of this injectisome is proposed to play an important role in energizing secretion events and substrate recognition. We present the 3.3 Å resolution cryo-EM structure of the enteropathogenic Escherichia coli ATPase EscN in complex with its central stalk EscO. The structure shows an asymmetric pore with different functional states captured in its six catalytic sites, details directly supporting a rotary catalytic mechanism analogous to that of the heterohexameric F/V-ATPases despite its homohexameric nature. Situated at the C-terminal opening of the EscN pore is one molecule of EscO, with primary interaction mediated through an electrostatic interface. The EscN-EscO structure provides significant atomic insights into how the ATPase contributes to type III secretion, including torque generation and binding of chaperone/substrate complexes.
External linksNat Commun / PubMed:30733444 / PubMed Central
MethodsEM (single particle)
Resolution3.29 - 3.34 Å
Structure data

9390

6njo

EMDB-9390, PDB-6njo:
Structure of the assembled ATPase EscN from the enteropathogenic E. coli (EPEC) type III secretion system
Method: EM (single particle) / Resolution: 3.34 Å

9391

6njp

EMDB-9391, PDB-6njp:
Structure of the assembled ATPase EscN in complex with its central stalk EscO from the enteropathogenic E. coli (EPEC) type III secretion system
Method: EM (single particle) / Resolution: 3.29 Å

Chemicals

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

ChemComp-AF3:
ALUMINUM FLUORIDE

ChemComp-HOH:
WATER

Source
  • Escherichia coli O127:H6 str. E2348/69 (bacteria)
  • escherichia coli o127:h6 (strain e2348/69 / epec) (bacteria)
KeywordsHYDROLASE / ATPase / Type III Secretion System / ADP / hexamer

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