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-Structure paper
| タイトル | Structure Reveals a Mechanism of CRISPR-RNA-Guided Nuclease Recruitment and Anti-CRISPR Viral Mimicry. |
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| ジャーナル・号・ページ | Mol Cell, Vol. 74, Issue 1, Page 132-142.e5, Year 2019 |
| 掲載日 | 2019年4月4日 |
著者 | MaryClare F Rollins / Saikat Chowdhury / Joshua Carter / Sarah M Golden / Heini M Miettinen / Andrew Santiago-Frangos / Dominick Faith / C Martin Lawrence / Gabriel C Lander / Blake Wiedenheft / ![]() |
| PubMed 要旨 | Bacteria and archaea have evolved sophisticated adaptive immune systems that rely on CRISPR RNA (crRNA)-guided detection and nuclease-mediated elimination of invading nucleic acids. Here, we present ...Bacteria and archaea have evolved sophisticated adaptive immune systems that rely on CRISPR RNA (crRNA)-guided detection and nuclease-mediated elimination of invading nucleic acids. Here, we present the cryo-electron microscopy (cryo-EM) structure of the type I-F crRNA-guided surveillance complex (Csy complex) from Pseudomonas aeruginosa bound to a double-stranded DNA target. Comparison of this structure to previously determined structures of this complex reveals a ∼180-degree rotation of the C-terminal helical bundle on the "large" Cas8f subunit. We show that the double-stranded DNA (dsDNA)-induced conformational change in Cas8f exposes a Cas2/3 "nuclease recruitment helix" that is structurally homologous to a virally encoded anti-CRISPR protein (AcrIF3). Structural homology between Cas8f and AcrIF3 suggests that AcrIF3 is a mimic of the Cas8f nuclease recruitment helix. |
リンク | Mol Cell / PubMed:30872121 / PubMed Central |
| 手法 | EM (単粒子) |
| 解像度 | 3.4 Å |
| 構造データ | |
| 由来 |
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キーワード | IMMUNE SYSTEM/RNA / type I-F CRISPR RNA-guided surveillance complex / viral protein mimic / Csy-complex / IMMUNE SYSTEM-RNA complex |
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Pseudomonas aeruginosa (strain UCBPP-PA14) (緑膿菌)
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