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Structure paper

TitleIsoprenoid-chained lipid EROCOC 17+4 : a new matrix for membrane protein crystallization and a crystal delivery medium in serial femtosecond crystallography.
Journal, issue, pagesSci Rep, Vol. 10, Page 19305-19305, Year 2020
Publish dateJan 10, 2020 (structure data deposition date)
AuthorsIhara, K. / Hato, M. / Nakane, T. / Yamashita, K. / Kimura-Someya, T. / Hosaka, T. / Ishizuka-Katsura, Y. / Tanaka, R. / Tanaka, T. / Sugahara, M. ...Ihara, K. / Hato, M. / Nakane, T. / Yamashita, K. / Kimura-Someya, T. / Hosaka, T. / Ishizuka-Katsura, Y. / Tanaka, R. / Tanaka, T. / Sugahara, M. / Hirata, K. / Yamamoto, M. / Nureki, O. / Tono, K. / Nango, E. / Iwata, S. / Shirouzu, M.
External linksSci Rep / PubMed:33168855
MethodsX-ray diffraction
Resolution1.8 - 2 Å
Structure data

PDB-6lpj:
A2AR crystallized in EROCOC17+4, LCP-SFX at 277 K
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-6lpk:
A2AR crystallized in EROCOC17+4, LCP-SFX at 293 K
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-6lpl:
A2AR crystallized in EROCOC17+4, SS-ROX at 100 K
Method: X-RAY DIFFRACTION / Resolution: 2 Å

Chemicals

ChemComp-ZMA:
4-{2-[(7-amino-2-furan-2-yl[1,2,4]triazolo[1,5-a][1,3,5]triazin-5-yl)amino]ethyl}phenol / antagonist*YM

ChemComp-NA:
Unknown entry

ChemComp-CLR:
CHOLESTEROL

ChemComp-D12:
DODECANE

ChemComp-MYS:
PENTADECANE

ChemComp-HEX:
HEXANE

ChemComp-8K6:
Octadecane

ChemComp-D10:
DECANE

ChemComp-OCT:
N-OCTANE

ChemComp-UND:
UNDECANE

ChemComp-ER0:
[(2~{R},3~{S})-2,3,4-tris(oxidanyl)butyl] (5~{R},9~{R},13~{R})-5,9,13,17-tetramethyloctadecanoate

ChemComp-TRD:
TRIDECANE

ChemComp-HOH:
WATER

Source
  • homo sapiens (human)
  • escherichia coli (E. coli)
KeywordsMEMBRANE PROTEIN / LCP crystallization / Isoprenoid-Chained-Lipid / GPCR / SFX / SS-ROX

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