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TitleCryo-EM structures of the air-oxidized and dithionite-reduced photosynthetic alternative complex III from .
Journal, issue, pagesSci Adv, Vol. 6, Issue 31, Page eaba2739, Year 2020
Publish dateJul 29, 2020
AuthorsYang Shi / Yueyong Xin / Chao Wang / Robert E Blankenship / Fei Sun / Xiaoling Xu /
PubMed AbstractAlternative complex III (ACIII) is a multisubunit quinol:electron acceptor oxidoreductase that couples quinol oxidation with transmembrane proton translocation in both the respiratory and ...Alternative complex III (ACIII) is a multisubunit quinol:electron acceptor oxidoreductase that couples quinol oxidation with transmembrane proton translocation in both the respiratory and photosynthetic electron transport chains of bacteria. The coupling mechanism, however, is poorly understood. Here, we report the cryo-EM structures of air-oxidized and dithionite-reduced ACIII from the photosynthetic bacterium at 3.3- and 3.5-Å resolution, respectively. We identified a menaquinol binding pocket and an electron transfer wire comprising six hemes and four iron-sulfur clusters that is capable of transferring electrons to periplasmic acceptors. We detected a proton translocation passage in which three strictly conserved, mid-passage residues are likely essential for coupling the redox-driven proton translocation across the membrane. These results allow us to propose a previously unrecognized coupling mechanism that links the respiratory and photosynthetic functions of ACIII. This study provides a structural basis for further investigation of the energy transformation mechanisms in bacterial photosynthesis and respiration.
External linksSci Adv / PubMed:32832681 / PubMed Central
MethodsEM (single particle)
Resolution3.2 - 3.5 Å
Structure data

0936

6lod

EMDB-0936, PDB-6lod:
Cryo-EM structure of the air-oxidized photosynthetic alternative complex III from Roseiflexus castenholzii
Method: EM (single particle) / Resolution: 3.2 Å

0937

6loe

EMDB-0937, PDB-6loe:
Cryo-EM structure of the dithionite-reduced photosynthetic alternative complex III from Roseiflexus castenholzii
Method: EM (single particle) / Resolution: 3.5 Å

Chemicals

ChemComp-HEC:
HEME C

ChemComp-EL6:
[(2S)-2-octadecanoyloxypropyl] octadecanoate

ChemComp-SF4:
IRON/SULFUR CLUSTER

ChemComp-F3S:
FE3-S4 CLUSTER

Source
  • roseiflexus castenholzii (strain dsm 13941 / hlo8) (bacteria)
KeywordsPHOTOSYNTHESIS / quinol:electron acceptor oxidoreductase

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