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Structure paper

TitleResurrecting the ancient glow of the fireflies.
Journal, issue, pagesSci Adv, Vol. 6, Year 2020
Publish dateMay 23, 2019 (structure data deposition date)
AuthorsOba, Y. / Konishi, K. / Yano, D. / Shibata, H. / Kato, D. / Shirai, T.
External linksSci Adv / PubMed:33268373
MethodsX-ray diffraction
Resolution1.7 - 2.1 Å
Structure data

PDB-6k4c:
Ancestral luciferase AncLamp in complex with DLSA
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-6k4d:
Ancestral luciferase AncLamp in complex with ATP and D-luciferin
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

Chemicals

ChemComp-SLU:
5'-O-[N-(DEHYDROLUCIFERYL)-SULFAMOYL] ADENOSINE

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-D4F:
[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] (4S)-2-(6-oxidanyl-1,3-benzothiazol-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylate

ChemComp-ESJ:
(4S)-2-(6-hydroxy-1,3-benzothiazol-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid

Source
  • lampyridae (fireflies)
KeywordsOXIDOREDUCTASE / Luciferase / Bioluminescence / Ancestral protein / Molecular evolution

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