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TitleStructure of Mutant Hepatitis B Core Protein Capsids with Premature Secretion Phenotype.
Journal, issue, pagesJ Mol Biol, Vol. 430, Issue 24, Page 4941-4954, Year 2018
Publish dateDec 7, 2018
AuthorsBettina Böttcher / Michael Nassal /
PubMed AbstractHepatitis B virus is a major human pathogen that consists of a viral genome surrounded by an icosahedrally ordered core protein and a polymorphic, lipidic envelope that is densely packed with surface ...Hepatitis B virus is a major human pathogen that consists of a viral genome surrounded by an icosahedrally ordered core protein and a polymorphic, lipidic envelope that is densely packed with surface proteins. A point mutation in the core protein in which a phenylalanine at position 97 is exchanged for a smaller leucine leads to premature envelopment of the capsid before the genome maturation is fully completed. We have used electron cryo-microscopy and image processing to investigate how the point mutation affects the structure of the capsid at 2.6- to 2.8 Å-resolution. We found that in the mutant the smaller side chain at position 97 is displaced, increasing the size of an adjacent pocket in the center of the spikes of the capsid. In the mutant, this pocket is filled with an unknown density. Phosphorylation of serine residues in the unresolved C-terminal domain of the mutant leaves the structure of the ordered capsid largely unchanged. However, we were able to resolve several previously unresolved residues downstream of proline 144 that precede the phosphorylation-sites. These residues pack against the neighboring subunits and increase the inter-dimer contact suggesting that the C-termini play an important role in capsid stabilization and provide a much larger interaction interface than previously observed.
External linksJ Mol Biol / PubMed:30539760
MethodsEM (single particle)
Resolution2.64 - 2.8 Å
Structure data

0271

6htx

EMDB-0271, PDB-6htx:
WT Hepatitis B core protein capsid
Method: EM (single particle) / Resolution: 2.66 Å

0272

6hu4

EMDB-0272, PDB-6hu4:
F97L Hepatitis B core protein capsid
Method: EM (single particle) / Resolution: 2.64 Å

0273

6hu7

EMDB-0273, PDB-6hu7:
phosphorylated F97L Hepatitis B core protein capsid
Method: EM (single particle) / Resolution: 2.8 Å

Source
  • hepatitis b virus ayw/france/tiollais/1979
  • hepatitis b virus
KeywordsVIRUS LIKE PARTICLE / Hapatitis B core protein Hbc Hbcag / VIRUS LIKE PARTICLE premature envelopment mutant F97L / VIRUS LIKE PARTICLE premature envelopment mutant phosphorylated F97L

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