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Structure paper

TitleStructural Insights into the Forward and Reverse Enzymatic Reactions in Human Adenine Phosphoribosyltransferase.
Journal, issue, pagesCell Chem Biol, Vol. 25, Page 666-676.e4, Year 2018
Publish dateDec 20, 2017 (structure data deposition date)
AuthorsHuyet, J. / Ozeir, M. / Burgevin, M.C. / Pinson, B. / Chesney, F. / Remy, J.M. / Siddiqi, A.R. / Lupoli, R. / Pinon, G. / Saint-Marc, C. ...Huyet, J. / Ozeir, M. / Burgevin, M.C. / Pinson, B. / Chesney, F. / Remy, J.M. / Siddiqi, A.R. / Lupoli, R. / Pinon, G. / Saint-Marc, C. / Gibert, J.F. / Morales, R. / Ceballos-Picot, I. / Barouki, R. / Daignan-Fornier, B. / Olivier-Bandini, A. / Auge, F. / Nioche, P.
External linksCell Chem Biol / PubMed:29576532
MethodsX-ray diffraction
Resolution1.45 - 1.94 Å
Structure data

PDB-6fch:
Crystal Structure of Human APRT wild type in complex with PRPP and Mg2+
Method: X-RAY DIFFRACTION / Resolution: 1.45 Å

PDB-6fci:
Crystal Structure of Human APRT wild type in complex with Adenine, PRPP and Mg2+
Method: X-RAY DIFFRACTION / Resolution: 1.94 Å

PDB-6fcl:
Crystal Structure of Human APRT wild type in complex with AMP
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-6fd4:
Crystal Structure of Human APRT-Tyr105Phe variant in complex with Adenine, PRPP and Mg2+, 14 hours post crystallization
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-6fd5:
Crystal Structure of Human APRT-Tyr105Phe variant in complex with Adenine, PRPP and Mg2+, 14 days post crystallization (with AMP and PPi products partially generated)
Method: X-RAY DIFFRACTION / Resolution: 1.55 Å

PDB-6fd6:
Crystal Structure of Human APRT-Tyr105Phe variant in complex with Adenine, PRPP and Mg2+, 30 days post crystallization (with AMP and PPi products fully generated)
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-PRP:
1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose

ChemComp-HOH:
WATER

ChemComp-ADE:
ADENINE

ChemComp-AMP:
ADENOSINE MONOPHOSPHATE / AMP*YM

ChemComp-PPV:
PYROPHOSPHATE

ChemComp-GOL:
GLYCEROL

Source
  • homo sapiens (human)
KeywordsTRANSFERASE / Rossman fold

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