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TitleSub-2 Å Ewald curvature corrected structure of an AAV2 capsid variant.
Journal, issue, pagesNat Commun, Vol. 9, Issue 1, Page 3628, Year 2018
Publish dateSep 7, 2018
AuthorsYong Zi Tan / Sriram Aiyer / Mario Mietzsch / Joshua A Hull / Robert McKenna / Joshua Grieger / R Jude Samulski / Timothy S Baker / Mavis Agbandje-McKenna / Dmitry Lyumkis /
PubMed AbstractSingle-particle cryogenic electron microscopy (cryo-EM) provides a powerful methodology for structural biologists, but the resolutions typically attained with experimentally determined structures ...Single-particle cryogenic electron microscopy (cryo-EM) provides a powerful methodology for structural biologists, but the resolutions typically attained with experimentally determined structures have lagged behind microscope capabilities. Here, we exploit several technical advances to improve resolution, including per-particle contrast transfer function (CTF) refinement and correction for Ewald sphere curvature. The latter is demonstrated with several experimental samples and should become more standard as resolutions increase or at lower microscope accelerating voltages. The combined application of the described methods to micrographs recorded on a Titan Krios enables structure determination at ~1.86-Å resolution of an adeno-associated virus serotype 2 variant (AAV2), an important gene-delivery vehicle. The resulting structural details provide an improved model for understanding the biology of AAV that will guide future vector development for gene therapy.
External linksNat Commun / PubMed:30194371 / PubMed Central
MethodsEM (single particle)
Resolution1.86 Å
Structure data

9012

6e9d

EMDB-9012: Sub-2 Angstrom Ewald Curvature Corrected Single-Particle Cryo-EM Reconstruction of AAV-2 L336C
PDB-6e9d: Sub-2 Angstrom Ewald Curvature-Corrected Single-Particle Cryo-EM Reconstruction of AAV-2 L336C
Method: EM (single particle) / Resolution: 1.86 Å

Chemicals

ChemComp-HOH:
WATER / Water

Source
  • adeno-associated virus 2 srivastava/1982
KeywordsVIRUS LIKE PARTICLE / AAV-2 L336C / Gene therapy / Ewald sphere curvature correction / Per particle CTF

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