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TitleThe Structure and Dynamics of C. elegans Tubulin Reveals the Mechanistic Basis of Microtubule Growth.
Journal, issue, pagesDev Cell, Vol. 47, Issue 2, Page 191-204.e8, Year 2018
Publish dateOct 22, 2018
AuthorsSami Chaaban / Shashank Jariwala / Chieh-Ting Hsu / Stefanie Redemann / Justin M Kollman / Thomas Müller-Reichert / David Sept / Khanh Huy Bui / Gary J Brouhard /
PubMed AbstractThe dynamic instability of microtubules is a conserved and fundamental mechanism in eukaryotes. Yet microtubules from different species diverge in their growth rates, lattice structures, and ...The dynamic instability of microtubules is a conserved and fundamental mechanism in eukaryotes. Yet microtubules from different species diverge in their growth rates, lattice structures, and responses to GTP hydrolysis. Therefore, we do not know what limits microtubule growth, what determines microtubule structure, or whether the mechanisms of dynamic instability are universal. Here, we studied microtubules from the nematode C. elegans, which have strikingly fast growth rates and non-canonical lattices in vivo. Using a reconstitution approach, we discovered that C. elegans microtubules combine intrinsically fast growth with very frequent catastrophes. We solved the structure of C. elegans microtubules to 4.8 Å and discovered sequence divergence in the lateral contact loops, one of which is ordered in C. elegans but unresolved in other species. We provide direct evidence that C. elegans tubulin has a higher free energy in solution and propose a model wherein the ordering of lateral contact loops activates tubulin for growth.
External linksDev Cell / PubMed:30245157
MethodsEM (single particle)
Resolution4.8 Å
Structure data

9004

6e88

EMDB-9004, PDB-6e88:
Cryo-EM structure of C. elegans GDP-microtubule
Method: EM (single particle) / Resolution: 4.8 Å

Chemicals

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

Source
  • caenorhabditis elegans (invertebrata)
KeywordsSTRUCTURAL PROTEIN / Cytoskeletal protein

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