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Structure paper

TitleStructural basis of the filamin A actin-binding domain interaction with F-actin.
Journal, issue, pagesNat Struct Mol Biol, Vol. 25, Issue 10, Page 918-927, Year 2018
Publish dateSep 17, 2018
AuthorsDaniel V Iwamoto / Andrew Huehn / Bertrand Simon / Clotilde Huet-Calderwood / Massimiliano Baldassarre / Charles V Sindelar / David A Calderwood /
PubMed AbstractActin-cross-linking proteins assemble actin filaments into higher-order structures essential for orchestrating cell shape, adhesion, and motility. Missense mutations in the tandem calponin homology ...Actin-cross-linking proteins assemble actin filaments into higher-order structures essential for orchestrating cell shape, adhesion, and motility. Missense mutations in the tandem calponin homology domains of their actin-binding domains (ABDs) underlie numerous genetic diseases, but a molecular understanding of these pathologies is hampered by the lack of high-resolution structures of any actin-cross-linking protein bound to F-actin. Here, taking advantage of a high-affinity, disease-associated mutant of the human filamin A (FLNa) ABD, we combine cryo-electron microscopy and functional studies to reveal at near-atomic resolution how the first calponin homology domain (CH1) and residues immediately N-terminal to it engage actin. We further show that reorientation of CH2 relative to CH1 is required to avoid clashes with actin and to expose F-actin-binding residues on CH1. Our data explain localization of disease-associated loss-of-function mutations to FLNaCH1 and gain-of-function mutations to the regulatory FLNaCH2. Sequence conservation argues that this provides a general model for ABD-F-actin binding.
External linksNat Struct Mol Biol / PubMed:30224736 / PubMed Central
MethodsEM (helical sym.)
Resolution3.54 - 9.8 Å
Structure data

7831

6d8c

EMDB-7831, PDB-6d8c:
Cryo-EM structure of FLNaABD E254K bound to phalloidin-stabilized F-actin
Method: EM (helical sym.) / Resolution: 3.54 Å

EMDB-7832:
FLNaABD-Q170P bound to phalloidin-stabilized F-actin
Method: EM (helical sym.) / Resolution: 6.6 Å

EMDB-7833:
FLNaABD-WT bound to phalloidin-stabilized F-actin
Method: EM (helical sym.) / Resolution: 9.8 Å

EMDB-8918:
Cryo-EM structure of FLNa ABD-E254K bound to phalloidin-stabilized F-actin, collected on a FEI Tecnai F20 microscope
Method: EM (helical sym.) / Resolution: 7.4 Å

Chemicals

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

Source
  • homo sapiens (human)
  • gallus gallus (chicken)
  • amanita phalloides (death cap)
KeywordsSTRUCTURAL PROTEIN / Actin-binding domain / Actin crosslinker

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