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TitleStructural visualization of RNA polymerase III transcription machineries.
Journal, issue, pagesCell Discov, Vol. 4, Page 40, Year 2018
Publish dateJul 31, 2018
AuthorsYan Han / Chunli Yan / Susan Fishbain / Ivaylo Ivanov / Yuan He /
PubMed AbstractRNA polymerase III (Pol III) transcription initiation requires the action of the transcription factor IIIB (TFIIIB) and is highly regulated. Here, we determine the structures of Pol III pre- ...RNA polymerase III (Pol III) transcription initiation requires the action of the transcription factor IIIB (TFIIIB) and is highly regulated. Here, we determine the structures of Pol III pre-initiation complexes (PICs) using single particle cryo-electron microscopy (cryo-EM). We observe stable Pol III-TFIIIB complexes using nucleic acid scaffolds mimicking various functional states, in which TFIIIB tightly encircles the upstream promoter DNA. There is an intricate interaction between TFIIIB and Pol III, which stabilizes the winged-helix domains of the C34 subunit of Pol III over the active site cleft. The architecture of Pol III PIC more resembles that of the Pol II PIC than the Pol I PIC. In addition, we also obtain a 3D reconstruction of Pol III in complex with TFIIIB using the elongation complex (EC) scaffold, shedding light on the mechanism of facilitated recycling of Pol III prior to transcription re-initiation.
External linksCell Discov / PubMed:30083386 / PubMed Central
MethodsEM (single particle)
Resolution4.1 - 8.5 Å
Structure data

7530

6cnb

EMDB-7530, PDB-6cnb:
Yeast RNA polymerase III initial transcribing complex
Method: EM (single particle) / Resolution: 4.1 Å

7531

6cnc

EMDB-7531, PDB-6cnc:
Yeast RNA polymerase III open complex
Method: EM (single particle) / Resolution: 4.1 Å

7532

6cnd

EMDB-7532, PDB-6cnd:
Yeast RNA polymerase III natural open complex (nOC)
Method: EM (single particle) / Resolution: 4.8 Å

7533

6cnf

EMDB-7533, PDB-6cnf:
Yeast RNA polymerase III elongation complex
Method: EM (single particle) / Resolution: 4.5 Å

EMDB-7534:
Yeast RNA polymerase III intermediate open complex (iOC)
Method: EM (single particle) / Resolution: 8.5 Å

Chemicals

ChemComp-ZN:
Unknown entry

Source
  • saccharomyces cerevisiae (strain atcc 204508 / s288c) (yeast)
  • Baker's yeast (brewer's yeast)
  • saccharomyces cerevisiae s288c (yeast)
Keywordstranscription/dna / RNA polymerase III / TFIIIB / tRNA / TRANSCRIPTION / transcription-dna complex

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