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TitleOrganometallic and radical intermediates reveal mechanism of diphthamide biosynthesis.
Journal, issue, pagesScience, Vol. 359, Page 1247-1250, Year 2018
Publish dateDec 18, 2017 (structure data deposition date)
AuthorsDong, M. / Kathiresan, V. / Fenwick, M.K. / Torelli, A.T. / Zhang, Y. / Caranto, J.D. / Dzikovski, B. / Sharma, A. / Lancaster, K.M. / Freed, J.H. ...Dong, M. / Kathiresan, V. / Fenwick, M.K. / Torelli, A.T. / Zhang, Y. / Caranto, J.D. / Dzikovski, B. / Sharma, A. / Lancaster, K.M. / Freed, J.H. / Ealick, S.E. / Hoffman, B.M. / Lin, H.
External linksScience / PubMed:29590073
MethodsX-ray diffraction
Resolution1.655 - 2.347 Å
Structure data

PDB-6bxk:
Crystal structure of Pyrococcus horikoshii Dph2 with 4Fe-4S cluster and MTA
Method: X-RAY DIFFRACTION / Resolution: 2.347 Å

PDB-6bxl:
Crystal structure of Pyrococcus horikoshii Dph2 with 4Fe-4S cluster and SAM
Method: X-RAY DIFFRACTION / Resolution: 2.301 Å

PDB-6bxm:
Crystal structure of Candidatus Methanoperedens nitroreducens Dph2 with 4Fe-4S cluster and SAM/cleaved SAM
Method: X-RAY DIFFRACTION / Resolution: 2.252 Å

PDB-6bxn:
Crystal structure of Candidatus Methanoperedens nitroreducens Dph2 with 4Fe-4S cluster and SAM
Method: X-RAY DIFFRACTION / Resolution: 2.079 Å

PDB-6bxo:
Crystal structure of Candidatus Methanoperedens nitroreducens Dph2 with 4Fe-4S cluster and SAH
Method: X-RAY DIFFRACTION / Resolution: 1.655 Å

Chemicals

ChemComp-MTA:
5'-DEOXY-5'-METHYLTHIOADENOSINE

ChemComp-SO4:
SULFATE ION

ChemComp-SF4:
IRON/SULFUR CLUSTER

ChemComp-HOH:
WATER

ChemComp-SAM:
S-ADENOSYLMETHIONINE

ChemComp-ABA:
ALPHA-AMINOBUTYRIC ACID

ChemComp-SAH:
S-ADENOSYL-L-HOMOCYSTEINE

Source
  • pyrococcus horikoshii (strain atcc 700860 / dsm 12428 / jcm 9974 / nbrc 100139 / ot-3) (archaea)
  • candidatus methanoperedens nitroreducens (archaea)
KeywordsBIOSYNTHETIC PROTEIN / DIPHTHAMIDE BIOSYNTHESIS / RADICAL SAM ENZYME

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