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TitleDeterminants and Expansion of Specificity in a Trichothecene UDP-Glucosyltransferase from Oryza sativa.
Journal, issue, pagesBiochemistry, Vol. 56, Page 6585-6596, Year 2017
Publish dateNov 7, 2017 (structure data deposition date)
AuthorsWetterhorn, K.M. / Gabardi, K. / Michlmayr, H. / Malachova, A. / Busman, M. / McCormick, S.P. / Berthiller, F. / Adam, G. / Rayment, I.
External linksBiochemistry / PubMed:29140092
MethodsX-ray diffraction
Resolution1.47 - 2.17 Å
Structure data

PDB-6bk0:
Crystal structure of Os79 Q202A from O. sativa in complex with UDP.
Method: X-RAY DIFFRACTION / Resolution: 1.47 Å

PDB-6bk1:
Crystal structure of Os79 T291V from O. sativa in complex with UDP.
Method: X-RAY DIFFRACTION / Resolution: 1.58 Å

PDB-6bk2:
Crystal structure of Os79 H122A/L123A from O. sativa in complex with UDP.
Method: X-RAY DIFFRACTION / Resolution: 1.68 Å

PDB-6bk3:
Crystal structure of Os79 from O. sativa in complex with UDP and deoxynivalenol-3-glucoside (glucose moitey not resolved)
Method: X-RAY DIFFRACTION / Resolution: 2.17 Å

Chemicals

ChemComp-UDP:
URIDINE-5'-DIPHOSPHATE / UDP*YM

ChemComp-HOH:
WATER

ChemComp-B2S:
(3alpha,7alpha)-3,7,15-trihydroxy-12,13-epoxytrichothec-9-en-8-one

Source
  • oryza sativa subsp. japonica (Japanese rice)
  • oryza sativa japonica group (Japanese rice)
KeywordsTRANSFERASE / UDP-glucosyltransferase / trichothecene

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